biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact us
 


Technical Notes
 
View our tech notes
 
 
Follow us...
 
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive
 
 

Beta-Amyloid Abeta1-42 Peptide, HFIP-treated

$97.00USD


Catalogue No. PE-1749-50
Description Synthetic beta-amyloid Aβ1-42 was monomerized by HFIP (hexafluoro-2-propanol) treatment and dried. One vial contains 50 g monomeric Aβ peptide that can be used to form solutions of unaggregated Aβ monomers, aggregated Aβ oligomers, Aβ fibrils and Aβ protein complexes according to published protocols, and used in a variety of research applications.
Related products Oligomeric Aβ ELISA Kit (BEK-2215)
ApoE/Aβ Complex ELISA kit (BEK-2224)
Oligomeric Aβ Peptide, stabilized (PE-1750-1000)
MOAB-2 Mouse Monoclonal Antibody to Aβ Peptide (Aβ1-40/42, M-1586-100)
Batch No. See product label. Each lot is tested by ELISA assay and validated for oligomer formation (Oligomeric Aβ ELISA Kit, cat# BEK-2215).
Unit size 3 vials. 1 x 50 g Abeta1-42 peptide; 1 x 100 L Reconstituting Buffer, 1 x 1 mL Dilution Buffer
Sequence DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
Other Names Beta-APP42; Beta-amyloid protein 42; ABPP; APPI; Amyloid beta A4 protein; AB42; abeta
Accession P05067 A4_HUMAN;
Produced in Synthetic
Molecular Weight 4.5 kDa (monomer)
Applications Preparation of unaggregated Aβ1-42:

Important: unaggregated Aβ has to be prepared just prior to use!

1. Add 5 L of reconstituting buffer to one vial of 50g of HFIP-treated Aβ peptide; spin down the liquid briefly
2. Vortex the vial for 5 seconds at highest speed while rotating the vial with your hands; spin down the liquid (bench-top microcentrifuge) and repeat the vortex-spin procedure for a minimum of 3 times; continue the vortex-spin procedure until all lyophilized peptide is dissolved and collected at the bottom of the tube. Important: refer to the attached instructions for a detailed procedure to ensure that all peptide is fully reconstituted!
3. Add 106 L of cold Dilution Buffer to make up to 111L total volume and a peptide concentration of 100 M. Vortex-spin for 3 more times
4. Final concentration of Aβ is 450 g/mL
5. Use reconstituted peptide immediately to avoid oligomer formation

Preparation of oligomeric Aβ1-42:

1. Add 5 L of reconstituting buffer to one vial of 50g of HFIP-treated Aβ peptide; spin down the liquid briefly
2. Vortex the vial for 5 seconds at highest speed while rotating the vial with your hands; spin down the liquid (bench-top microcentrifuge) and repeat the vortex-spin procedure for a minimum of 3 times; continue the vortex-spin procedure until all lyophilized peptide is dissolved and collected at the bottom of the tube. Important: refer to the attached instructions for a detailed procedure to ensure that all peptide is fully reconstituted!
3. Add 106 L of cold Dilution Buffer to make up to 111L total volume and a peptide concentration of 100 M
4. Vortex-spin for 3 more times
5. Incubate the solution at 2-8C for 24 hours (protected from light)
6. Final concentration of Aβ is 450 g/mL
7. Once reconstituted and oligomerized, o-Aβ should be used as soon as possible and within 7 days to ensure the stability of the oligomers

Note: while the concentration of monomeric Aβ peptide used to form the oligomeric complexes is accurately determined, the precise formation, size and number of oligomers cannot be quantified by any known method.

Preparation of fibrillar Aβ1-42:

1. Add 5 L of reconstituting buffer to one vial of 50g of HFIP-treated Aβ peptide; spin down the liquid briefly
2. Vortex the vial for 5 seconds at highest speed while rotating the vial with your hands; spin down the liquid (bench-top microcentrifuge) and repeat the vortex-spin procedure for a minimum of 3 times; continue the vortex-spin procedure until all lyophilized peptide is dissolved and collected at the bottom of the tube. Important: refer to the attached instructions for a detailed procedure to ensure that all peptide is fully reconstituted!
3. Add 106 L of 10 mM HCl to make up to 111L total volume and a peptide concentration of 100 M
4. Vortex-spin for 3 more times
5. Incubate the solution at 37C for 24 hours (protected from light)
6. Final concentration of Aβ is 450 g/mL

Preparation of Aβ1-42 Complexes:

Important: only unagreggated Aβ will form complexes. Use Aβ peptide immediately after reconstitution to form complexes.

1. Add 5 L of reconstituting buffer to one vial of 50g of HFIP-treated Aβ peptide; spin down the liquid briefly
2. Vortex the vial for 5 seconds at highest speed while rotating the vial with your hands; spin down the liquid (bench-top microcentrifuge) and repeat the vortex-spin procedure for a minimum of 3 times; continue the vortex-spin procedure until all lyophilized peptide is dissolved and collected at the bottom of the tube. Important: refer to the attached instructions for a detailed procedure to ensure that all peptide is fully reconstituted!
3. Add 106 L of cold Dilution Buffer to make up to 111L total volume and a peptide concentration of 100 M
4. Vortex-spin for 3 more times
5. Use reconstituted peptide immediately to avoid oligomer formation
6. Mix the Aβ monomer with its complex partner (eg., lipoprotein) at desired concentrations in PBS, pH 7.4, or other suitable buffers compatible with its intended application
7. Incubate at room temperature for 2 hours without shaking
8. Use complexes immediately after incubation

These protocols are based on procedures published by Youmans KL et al., 2012 and Tai LM et al., 2013, and we refer to these publications and other relevant literature for further details.

Provided working concentrations are only meant to guide the user. Optimal concentrations depend on the experimental design and need to be determined empirically.
Form Lyophilized.
Appearance Translucent film or droplets, dry, without preservatives.
Reconstitution Varies, dependent on intended use. Refer to Applications.
Storage Store unopened, dry Aβ peptide vial with desiccant, insulated, at -20C short term, -80C long term. Store the buffers at 2-8C, do not freeze.
Expiry Date Stability of unopened vials is 1 year if stored appropriately.
References Youmans KL. et al. (2012) Intraneuronal Abeta detection in 5xFAD mice by a new Abeta-specific antibody. Mol Neurodegener. 2012 Mar 16;7(1):8.

Tai LM. et al. (2013) Levels of Soluble Apolipoprotein E/Amyloid-beta (Aβ) Complex Are Reduced and Oligomeric Abeta Increased with APOE4 and Alzheimer Disease in a Transgenic Mouse Model and Human Samples. J Biol Chem. 2013 Feb 22;288(8):5914-26.
Images (click to zoom)
Beta-Amyloid Abeta1-42 Peptide, HFIP-treated Oligomeric A-beta standard curves generated with BEK-2215 (Oligomeric A beta ELISA kit). Pre-formed oligomers (PE-1750-1000) were reconstituted in assay buffer and compared to oligomers freshly prepared from HFIP-treated A-beta peptide (PE-1749-50). This data demonstrates the usefulness of PE-1749-50 as oligomeric A-beta protein standard.

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

Customers who bought this product also purchased
Oligomeric Human beta-Amyloid Abeta1-42 Peptide, Stabilized
Oligomeric Human beta-Amyloid Abeta1-42 Peptide, Stabilized
MOAB-2 Mouse Monoclonal antibody to Amyloid beta peptide (A beta 40/42), purified
MOAB-2 Mouse Monoclonal antibody to Amyloid beta peptide (A beta 40/42), purified

Offices in the USA and Australia
My Ice Bucket  
 
0 items
 
 
Currencies
 
  
 
 
Email This Page
 
 
Tell someone you know about this product.
 
 
We Accept PayPal
 
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment


Official PayPal Seal