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Biosensis ER-O Endoplasmic Reticulum Tracing Reagent

$420.00USD


Catalogue No. TR-601-ER1
Description TR-601-ER1 is a cell-permeant stain for the endoplasmic reticulum. TR-601-ER1 passively diffuses across the plasma membrane into the cell, stains at low concentrations and has minimal cytotoxic effects. It can be used as a real-time imaging reagent which can be imaged within minutes of addition and has minimal photobleaching. TR-601-ER1 is easily washed from cells, and therefore is ideal for protocols which require intermittent monitoring of endoplasmic reticulum structures. This tracing reagent can be used on live and fixed samples and is compatible with other dyes. TR-601-ER1 has a large Stokes Shift, shows excellent resistance to photobleaching and demonstrates low cytotoxicity. TR-601-ER1 is manufactured by ReZolve Scientific.
Batch No. Please refer to the product label.
Unit size 1.8 mg
Applications Fluorescent imaging of the endoplasmic reticulum compartment.

Sample types: live cells, fixed cells (4% paraformaldehyde), tissue (frozen only)
Epifluorescence microscopy: YES
Confocal microscopy: YES
Multiphoton imaging: YES
Infrared spectroscopy: NO
Raman spectroscopy: NO
Ex/Em: 405 nm / 570 nm

Typical working dilution is 1:100 - 1:200 (50-100 µM) in appropriate buffer or cell culture medium. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Cell penetration and localisation of TR-601-ER1 has been confirmed in a range of cell lines, including prostate cells (PNT2, PNT1a, LNCaP, 22RV1 and DU145), cardiomyocytes (H9c2) and neuronal cells (PC-12).
Reconstitution Spin vial briefly before opening. Reconstitute TR-601-ER1 with 300 µL of DMSO to obtain a 10 mM stock solution, mix thoroughly before use. Avoid PBS or cell culture medium for reconstitution.
Storage Product is shipped at room temperature. Store lyophilised product at room temperature, properly sealed and protected from light. Stock solution can be stored at room temperature, properly sealed and protected from light. Working solutions should be used immediately and not stored for later use.
Expiry Date Lyophilised product is stable for 6 months at room temperature if protected from light. After reconstitution in DMSO, liquid 10 mM stock solution is stable for 6 months at room temperature.
General References Bader CA et al. (2016) Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time. FEBS Lett. 2016 Sep;590(18):3051-60.
Reagent Kit protocol Click to download the protocolTR-601-ER1_as_at_Sep2018.pdf
MSDS Click to download the Material Safety Data SheetTR-601-ER1-SDS.pdf
Images (click to zoom)
Biosensis ER-O Endoplasmic Reticulum Tracing Reagent Comparison of TR-601-ER1 with ER-Tracker (upper panel). TR-601-ER1 can be used to stain the endoplasmic reticulum in live and fixed cells (lower panel).
Biosensis ER-O Endoplasmic Reticulum Tracing Reagent Staining of endoplasmic reticulum in cultured cells.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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