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Biosensis ER-B Endoplasmic Reticulum Tracing Reagent

$370.00USD


Catalogue No. TR-605-ER2
Description Biosensis ER-BTM Endoplasmic Reticulum Tracing Reagent is a cell-permeant stain for the endoplasmic reticulum with unique spectral properties, which makes it particularly useful for antibody double- and triple-labelling experiments. TR-605-ER2 is highly compatible with green, red and far-red fluorophore-labelled antibodies and has been successfully used to image the endoplasmic reticulum in human and other mammalian cell lines. TR-605-ER2 passively diffuses across the plasma membrane into the cell, stains at low concentrations and has minimal cytotoxic effects. This tracing reagent can be used on live and fixed samples, is compatible with other dyes, and shows excellent resistance to photobleaching. TR-605-ER2 is manufactured by ReZolve Scientific.
Related products Biosensis ER-O Endoplasmic Reticulum Tracing Reagent (Ex/Em = 405/570 nm), TR-601-ER1
Batch No. Please refer to the product label.
Unit size 1.0 mg
Applications Fluorescent imaging of the endoplasmic reticulum compartment.

Sample types: live cells, fixed cells (4% paraformaldehyde)
Microscopy applications: epifluorescence, confocal, multicolour
Ex/Em: 405 nm / 500 nm; TR-605-ER2 can be excited by UV (~365 nm) or blue light (405 nm) sources with emissions collected using a wideband pass filter, or narrowband pass filter with an emission range 450-570 nm (Emmax = 500 nm). For confocal microscopy, the dye can be excited by a 400 nm steady state laser, and emission should be collected using a detector suited to blue fluorophores such as DAPI. Alternatively, a spectral detector set for the emission of ER-BTM stain (450-570 nm, Emmax = 500 nm) can be used.

Typical working dilution is 1:1000 - 1:200 (10-50 uM) in appropriate buffer or cell culture medium. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Cell penetration and localisation of TR-605-ER2 has been confirmed in a range of mammalian cell lines (PNT2, 22RV1, H9c2).
Reconstitution Spin vial briefly before opening. Reconstitute TR-605-ER2 with 144 uL of DMSO to obtain a 10 mM stock solution, mix thoroughly before use. Avoid PBS or cell culture medium for reconstitution.
Storage Product is shipped at room temperature. Store lyophilised product at room temperature, properly sealed and protected from light. Stock solution can be stored at room temperature, properly sealed and protected from light. Working solutions should be used immediately and not stored for later use.
Expiry Date Lyophilised product is stable for 6 months at room temperature after purchase, if protected from light. After reconstitution in DMSO, liquid 10 mM stock solution is stable for 4 months at room temperature.
Reagent Kit protocol Click to download the protocolTR-605-ER2_as_at_Feb2019.pdf
Images (click to zoom)
Biosensis ER-B Endoplasmic Reticulum Tracing Reagent Staining of the endoplasmic reticulum (blue) in cell culture. Antibody co-staining reveals localisation of Cytochrome C (red).

PDF Data Sheet

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MSDS

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