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Biosensis Mito-R Mitochondria Tracing Reagent

$495.00USD


Catalogue No. TR-602-MR
Description TR-602-MR is a cell-permeant stain for mitochondria. This tracing reagent can be used on live cells, frozen and fixed tissue samples and is compatible with other dyes. TR-602-MR has a large Stokes Shift, shows excellent resistance to photobleaching and demonstrates low cytotoxicity. TR-602-MR is manufactured by ReZolve Scientific.
Batch No. Please refer to the product label.
Unit size 0.5 mg
Applications Fluorescent imaging of mitochondria.

Sample types: live cells, tissue (frozen and 4% paraformaldehyde). Quenching endogenous fluorescence can assist in imaging, refer to the product manual. Not suitable for application on fixed cells.
Epifluorescence microscopy: YES
Confocal microscopy: YES
Multiphoton imaging: YES
Infrared spectroscopy: NO
Raman spectroscopy: NO
Ex/Em: 405 nm / 600 nm

Typical working dilution is 1:200 - 1:1,000 (10-50 M) in appropriate buffer or cell culture medium. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Cell penetration and localisation of TR-602-MR has been confirmed in prostate cells (PNT2 and 22RV1), cardiomyocytes (H9c2) and cancer cell lines (HeLa). Mitochondrial localisation has been shown in live tissues, including live adipose tissue (sheep) and muscle tissue (sheep cardiac and skeletal) and frozen or PFA fixed muscle tissue (sheep skeletal).
Reconstitution Spin vial briefly before opening. Reconstitute TR-602-MR with 55 L of DMSO to obtain a 10 mM stock solution, mix thoroughly before use. Avoid PBS or cell culture medium for reconstitution.
Storage Product is shipped at room temperature. Store lyophilised product at room temperature, properly sealed and protected from light. Stock solution should be stored at 2-8C, properly sealed and protected from light. Working solutions should be used immediately and not stored for later use.
Expiry Date Lyophilised product is stable for 6 months at room temperature if protected from light. After reconstitution in DMSO, liquid 10 mM stock solution is stable for 2 months at 2-8C.
General References Sorvina A et al. (2018) Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex. Sci Rep. 2018 May 29;8(1):8191.
Reagent Kit protocol Click to download the protocolTR-602-MR_as_at_Jun2018.pdf
MSDS Click to download the Material Safety Data SheetTR-602-MR-SDS.pdf
Images (click to zoom)
Biosensis Mito-R Mitochondria Tracing Reagent Staining of mitochondria in cardiomyocytes (top) and HeLa cells (bottom) with TR-602-MR tracing reagent.
Biosensis Mito-R Mitochondria Tracing Reagent Staining of mitochondria in HeLa cells.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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