||TR-602-MR is a cell-permeant stain for mitochondria. This tracing reagent can be used on live cells, frozen and fixed tissue samples and is compatible with other dyes. TR-602-MR has a large Stokes Shift, shows excellent resistance to photobleaching and demonstrates low cytotoxicity. TR-602-MR is manufactured by ReZolve Scientific.
||Please refer to the product label.
||Fluorescent imaging of mitochondria.
Sample types: live cells, tissue (frozen and 4% paraformaldehyde). Quenching endogenous fluorescence can assist in imaging, refer to the product manual. Not suitable for application on fixed cells.
Epifluorescence microscopy: YES
Confocal microscopy: YES
Multiphoton imaging: YES
Infrared spectroscopy: NO
Raman spectroscopy: NO
Ex/Em: 405 nm / 600 nm
Typical working dilution is 1:200 - 1:1,000 (10-50 uM) in appropriate buffer or cell culture medium. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||Cell penetration and localisation of TR-602-MR has been confirmed in prostate cells (PNT2 and 22RV1), cardiomyocytes (H9c2) and cancer cell lines (HeLa). Mitochondrial localisation has been shown in live tissues, including live adipose tissue (sheep) and muscle tissue (sheep cardiac and skeletal) and frozen or PFA fixed muscle tissue (sheep skeletal).
||Spin vial briefly before opening. Reconstitute TR-602-MR with 55 uL of DMSO to obtain a 10 mM stock solution, mix thoroughly before use. Avoid PBS or cell culture medium for reconstitution.
||Store lyophilised product at 2-8C, properly sealed and protected from light. Stock solution should be stored at 2-8C, properly sealed and protected from light. Working solutions should be used immediately and not stored for later use.
||Lyophilised product is stable for 6 months at 2-8C if protected from light. After reconstitution in DMSO, liquid 10 mM stock solution is stable for 2 months at 2-8C.
||Sorvina A et al. (2018) Mitochondrial imaging in live or fixed tissues using a luminescent iridium complex. Sci Rep. 2018 May 29;8(1):8191.
|Reagent Kit protocol