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Biosensis P2 Polar Lipid and Endoplasmic Reticulum Tracing Reagent

$295.00USD


Catalogue No. TR-603-P2
Description TR-603-P2 is a cell-permeant stain for intracellular lipid droplets and the endoplasmic reticulum. This tracing reagent can be used on live and fixed cells and is compatible with other dyes. TR-603-P2 has a large Stokes Shift, shows excellent resistance to photobleaching and demonstrates low cytotoxicity. TR-603-P2 is manufactured by ReZolve Scientific.
Batch No. Please refer to the product label.
Unit size 1.0 mg
Applications Fluorescent imaging of lipid rich compartments.

Sample types: live and fixed (4% paraformaldehyde) cells. Serum-free culture medium is recommended for staining, as lipids contained in serum can reduce signal. Please refer to the product manual.
Epifluorescence microscopy: YES
Confocal microscopy: YES
Multiphoton imaging: YES
Infrared spectroscopy: NO
Raman spectroscopy: NO
Ex/Em: 405 nm / 600 nm

Typical working dilution is 1:500 - 1:1,000 (10-20 ÁM) in appropriate buffer or cell culture medium. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Cell penetration and localisation of TR-603-P2 has been confirmed in cardiomyocytes, H9c2 cell line and LNCaP prostate cells.
Reconstitution Spin vial briefly before opening. Reconstitute TR-603-P2 with 149 ÁL of DMSO to obtain a 10 mM stock solution, mix thoroughly before use. Avoid PBS or cell culture medium for reconstitution.
Storage Product is shipped at room temperature. Store lyophilised product at room temperature, properly sealed and protected from light. Stock solution can be stored at room temperature, properly sealed and protected from light. Working solutions should be used immediately and not stored for later use.
Expiry Date Lyophilised product is stable for 6 months at room temperature if protected from light. After reconstitution in DMSO, liquid 10 mM stock solution is stable for 6 months at room temperature.
Reagent Kit protocol Click to download the protocolTR-603-P2_as_at_Jun2018.pdf
MSDS Click to download the Material Safety Data SheetTR-603-P2-SDS.pdf
Images (click to zoom)
Biosensis P2 Polar Lipid and Endoplasmic Reticulum Tracing Reagent Staining in cultured cells with TR-603-P2 tracing reagent (Left: cardiomyocytes; Right: H9c2 cells).
Biosensis P2 Polar Lipid and Endoplasmic Reticulum Tracing Reagent Staining of cultured cells (Right: LNCaP prostate cancer cells)
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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