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Black-Gold II Myelin Ready-to-Dilute Staining Kit with Congo Red Counter Stain for identifying Myelin & Amyloid Plaques

$597.00USD


Catalogue No. TR-200-BG
Description
This product is no longer available. Please see below for our recommended alternative products.
Replaced by TR-100-BG
Other Names black and gold
Applications Black Gold II is a high resolution myelin stain with amyloid plaque counter stain. Its use is tailored to studies using formalin or paraformaldehyde fixed, non-paraffin embedded, non-solvent processed brain tissue. It can be used with both thick and thin sections. For thick sections, gelatin coated slides or slides specially designed to bind tissues sections should be use to avoid section loss. Free-floating sections can be used as well but sections are easier to handle and transfer when mounted on slides. A suggested method for thick sections is provided as a guide: Either frozen or vibratome sections are cut at a thickness of 20-50 um and collected in 0.1 M neutral phosphate buffer. The sections are then typically mounted on 1% gel-coated slides and then air dried on a slide warmer (at 50 degrees C) for at least an hour until throughly dried and adhered to the slide. The sections can be stained loose, although the sections are easier to handle when mounted on slides. The mounted sections were rehydrated in distilled water for 2 minutes before being processed in the staining solutions.
Form The reagents in the Black Gold kit are all supplied in a liquid format and are ready-to-dilute.
Storage The kit can be transported at room temperature. Once received, the kit can be stored for up to 12 months at 4C protected from light. Diluted solutions can be stored up to one month at 4C protected from light.
Kit Components Black-Gold II (Dilute 1:10 prior to use) - 10 mL Sodium Thiosulfate, fixative (Dilute 1:10 prior to use) - 10 mL Congo Red (Dilute 1:10 prior to use) - 10 mL Sodium Hydroxide (Dilute 1:10 prior to use) - 10 mL
Reagent Kit protocol Click to download the protocolTR-200-BG_April2012.pdf
MSDS Click to download the Material Safety Data SheetMSDS_TR-200-BG_BGT_as_at_Apr2014.pdf
Images (click to zoom)
Black-Gold II Myelin Ready-to-Dilute Staining Kit with Congo Red Counter Stain for identifying Myelin & Amyloid Plaques Black-Gold II staining reveals myelin pathology surrounding a Congo Red positive amyloid plaque within the dentate gyrus of an 8 month old AD-Tg mouse (60X mag). Photo is courtesy of Dr. Larry Schmued.
Black-Gold II Myelin Ready-to-Dilute Staining Kit with Congo Red Counter Stain for identifying Myelin & Amyloid Plaques Combined dark field and epi-fluorescent (green light excitation) of the hippocampus of an AD-Tg mouse. Staining reveals gold/lavender appearing myelinated fibres within the dentate gyrus. The granule cell layer remains unstained while conspicuous Congo red positive plaques exhibit an orange fluorescence in both the molecular and polymorph layers. Photo is courtesy of Dr. Larry Schmued.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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