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Bovine A1 Beta Casein ELISA Reagent Set (10 plates)

$2,177.00USD


Catalogue No. BEK-2364-SET10
Description Bovine milk may contain two types of beta-casein protein, A2 or A1, which differ by a single-point mutation at amino acid position 82 (P82/H82). Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.

The Biosensis Bovine A1 Beta Casein ELISA Kit is a sandwich ELISA that allows the specific quantification of the A1 isoform of bovine beta casein in bovine milk, only if used as directed. The A2 isoform is not detected in this ELISA assay. Please refer to the kit protocol for specific use instructions.

A matched pair of antibodies for capture and specific detection of A1 beta casein, native A1 protein standard and HRP-labelled secondary antibody is provided to coat and run 10 x 96-well microplates. Precise instructions are given to ensure consistent assay performance.
Related products BEK-2364-SET5, Bovine A1 Beta Casein ELISA Kit Bulk Pack (5 plates)
BEK-2364-1P, Bovine A1 Beta Casein ELISA Kit (1 plate)
Batch No. Refer to the product labels
Antigen Bovine A1 beta casein (H82)
Accession P02666 (CASB_BOVIN)
Applications ELISA. Bovine milk samples.
Specificity Bovine A1 beta casein. Other species not tested yet
Cross-reactivity No cross-reactivity is observed with bovine A2 beta casein
Storage Storage conditions are specific to individual kit components. Refer to the kit protocol for details.
Kit components Rabbit anti-bovine beta casein polyclonal capture antibody, 1 mg/mL, 500 ug (1 tube)
Chicken anti-bovine A1 beta casein polyclonal detection antibody, 0.5 mg/mL, 10 uL/tube (10 tubes)
Native bovine A1 beta-casein standard, 40 ug/mL, 20 uL/tube (10 tubes)
Rabbit anti-chicken IgY HRP-conjugate, 1000X, 20 uL/tube (10 tubes)

Reagents required, but not included in this kit:
96-well microplates
Coating buffer
TBS and TBST buffer
Tween-20
BSA
TMB substrate
1M HCl
0.5M NaOH
Range 6.25 ng/mL - 400 ng/mL
Kit protocol Please contact us at biospeak@biosensis.com for a detailed protocol for this ELISA kit.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

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