||Bovine milk contains two types of beta-casein protein, A2 or A1. Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.
|| C-1781-100: Chicken antibody to bovine A1 beta casein: Affinity purified.
R-1794-100: Rabbit antibody to bovine beta casein: Affinity purified.
||Please refer to the product label.
||100 ÁL (Refer to label for antibody concentration)
||A synthetic peptide (PGPIPNSLP, aa: 78-86) conjugated to KLH has been used as immunogen. Bovine A1 beta casein differs from bovine A2 beta casein by one amino acid (H82 -> P82)
||Affinity purified chicken IgY.
||Western blot and ELISA. Suggested working dilution for western blot is 1:200-1:1,000. Sample Preparation: Milk can be diluted 1:10 in 0.1M NaOH and then add the sample into SDS-Sample (dye) Buffer and boil for about 5 minutes. Recommended blocking buffer: TBS with 5% BSA. Recommended antibody dilution buffer: TBST containing 3% BSA. Biosensis recommends that optimal working dilutions should be determined by the end user.
||The antibody is specific to A2 beta casein by western blot and ELISA. No cross-reactivity with A1 beta casein is seen.
||Species cross-reactivity not tested.
||Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative. Refer to the product label for antibody concentration.
||Maintain unopened vial at -20degC for up to 12 months after date of receipt. After opening maintain at -20degC in undiluted aliquots for up to 6 months. For short-term storage, keep aliquot at 2-8degC for up to one week. Avoid repeated freeze-thaw cycles.
||12 months after date of receipt unopened and stored at -20degC.
||Nutr Diabetes. 2017 May 15;7(5):e274
Nutr J. 2016 Apr 2;15:35