biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact us
 


Technical Notes
 
View our tech notes
 
 
Follow us...
 
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive
 
 

Chicken antibody to human alpha synuclein: affinity purified

$297.00USD


Catalogue No. C-1795-50
Description May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Ref: uniprot.org.
Batch No. See product label.
Unit size 50 g
Antigen Full length human protein with the epitope from amino acids 61-95
Other Names Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
Accession P37840 (SYUA_HUMAN)
Produced in Chicken
Purity Affinity purified
Applications Western blotting (1:1,000 - 1:2,000), Immunocytochemistry (1:1,000 - 1:2,000) and Immunohistochemistry (1:1,000 1: 2,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Reacts with human, horse, cow, pig, chicken, rat, mouse.
Form Lyophilised from a solution containing PBS buffer pH 7.2-7.6 with 3% trehalose, 5 mM NaN3.
Reconstitution Spin vial briefly before opening. Reconstitute in 50 L sterile water. Centrifuge to remove any insoluble material. Final buffer contains preservatives.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Chicken antibody to human alpha synuclein: affinity purified Left: Rat cerebellum section stained with chicken anti-α-synuclein antibody (red, 1:3,000) by Immunohistochemistry. Section was co-stained with rabbit anti-GFAP (green, R-1374-50, 1:5,000) and DAPI nuclear dye (blue). IHC method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The α-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells. Right: Western blot analysis of tissue lysates. Lane 1: MWM; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord. Antibody dilution: 1:2,000. A strong band at about 15 kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses.
Chicken antibody to human alpha synuclein: affinity purified Left: Analysis of alpha-synuclein expression in mixed rat neuron-glial cultures by Immunocytochemistry. The alpha-synuclein antibody (red) stains vesicular structures the perikarya and processes of the neurons. The antibody reveals that some of the neuronal perikarya contain more alpha-synuclein than others. Green: MAP2, Blue: DNA. Right: Detection of alpha-synuclein monomer (~15 kDa) in crude rat brain extract by Western Blotting.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
 
0 items
 
 
Currencies
 
  
 
 
Email This Page
 
 
Tell someone you know about this product.
 
 
We Accept PayPal
 
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment


Official PayPal Seal