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Goat antibody to SOX2: affinity purified

$347.00USD


Catalogue No. G-1828-100
Description Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). Ref: uniprot.org
Batch No. See product label.
Unit size 100 ug
Antigen A synthetic peptide with sequence YNMMETELKPPGPQQ-C, corresponding to the N-terminus of SOX2 has been used as the immunogen.
Sequence YNMMETELKPPGPQQ-C
Antigen Location N-terminus
Antibody Type Polyclonal
Other Names SOX2; SRY (sex determining region Y)-box 2; ANOP3; MGC2413; SRY-related HMG-box gene 2; sex-determining region Y-box 2; transcription factor SOX2
Accession P48431 SOX2_HUMAN
Produced in Goat
Purity Affinity purified
Applications Western Blot (0.1-0.3 ug/mL) : A 37 kDa band is observed in nuclear lysates of cell line MCF7 and in lysates of human glioblastoma cell line U251. Immunocytochemistry (10 ug/mL): Strong expression of protein seen in the nuclei of U251 cells. Flow Cytometry (10 ug/mL): Analysis of MCF7 cells. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antibody is specific for SOX2 as demonstrated by western blotting.
Species Against Human
Antibody Against SOX2
Cross-reactivity Human. Other species not tested yet, but expected to cross-react with mouse, rat, dog and cow due to amino acid sequence similarity.
Form Lyophilized from a solution containing Tris-saline, 0.02% sodium azide, pH 7.3, with 0.5% bovine serum albumin.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile, ultrapure water to prepare 1 mg/mL solution. Centrifuge to remove any insoluble material.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Goat antibody to SOX2: affinity purified Left: Western Blot analysis of MCF7 nuclear (A) and U251 (B) cell lysate (35 ug/lane). Primary antibody was used at 0.3 ug/mL. Right: Analysis of SOX2 expression in paraformaldehyde-fixed MCF7 cells (blue line), permeabilized with 0.5% Triton, by Flow Cytometry. Primary antibody incubation with 10 ug/mL goat anti-SOX2 antibody for 1 hr followed by addition of fluorescent-labelled anti-goat secondary antibody (1 ug/mL). IgG control: Normal goat IgG (black line).
Goat antibody to SOX2: affinity purified Analysis of SOX2 expression (green) in paraformaldehyde-fixed U251 cells, permeabilized with 0.15% Triton, by Immunocytochemistry. Primary antibody: incubation with 10 ug/mL goat anti-SOX2 antibody for 1 hour, followed by addition of fluorescent-labelled secondary antibody (2 ug/mL). Actin filaments were stained with phalloidin (red). Negative control: Normal goat IgG (10 ug/mL). SOX2 expression is observed mainly in the nucleus of U251 cells.
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MSDS

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