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Heterophilic antibody blocker for BEK-2217, BEK-2221, BEK-2237, BEK-2240 and similar ELISA assays


Catalogue No. BL-004-500
Description BL-004-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing sheep immunoreagents.

Following ELISA assays in the Biosensis RapidTM ELISA range have been validated to achieve accurate results using BL-004-500:

BEK-2217/2240, Human proBDNF; Application: Serum, Citrate-Plasma
BEK-2221, Human NT3; Application: Plasma (Citrate and EDTA)
BEK-2237, Human proBDNF; Application: Serum, Plasma (Citrate and EDTA)

One vial of BL-004-500 contains 500 g IgG which is sufficient as sample diluent additive for one 96-well plate.

Other ELISA assays that use sheep assay antibodies may also benefit from addition of blocker BL-004-500, but require optimization of working concentration and assay validation for accurate results.
Related products BEK-2217-1P, Human proBDNF RapidTM ELISA
BEK-2217-2P, Human proBDNF RapidTM ELISA
BEK-2221-1P, Human NT3 RapidTM ELISA
BEK-2221-2P, Human NT3 RapidTM ELISA
BEK-2237-1P, Human proBDNF RapidTM ELISA
BEK-2237-2P, Human proBDNF RapidTM ELISA
BEK-2240, Mature BDNF/proBDNF Combo RapidTM ELISA
Batch No. See product label.
Unit size 500 g
Applications Immunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays for validated sample applications.
Form Lyophilized.
Appearance White powder.
Reconstitution 1) Reconstitute with 1 mL assay diluent buffer to achieve a blocker stock concentration of 500 g/mL.
2) Dilute this stock solution further with 24 mL assay diluent buffer to obtain the blocker working concentration of 20 g/mL. Refer to RapidTM ELISA kit protocol for further details.

The working concentration of this HA blocker needs to be optimized by the end-user for other ELISA assays.
Storage Store unopened vial at 2-8C.
Expiry Date 12 months after purchase (unopened vial).
Kit protocol Click to download the kit protocolBL-004-500_as_at_Dec2017.pdf
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet


Click to download the MSDS - Click to download the MSDS

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