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Human beta Nerve Growth Factor protein expressed in mammalian cells


Catalogue No. PE-1239-5
Description Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
Batch No. See product label
Unit size 5 ug
Other Names Beta-nerve growth factor; beta-NGF; NGFB;
Accession P01138 NGF_HUMAN;
Produced in Human - A DNA sequence encoding the human beta NGF protein sequence (containing the signal peptide, pro-peptide and the mature beta NGF sequence) was expressed in modified human 293 cells. Theoretical peptide sequence: YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEAR PVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT
Molecular Weight The NGF protein migrates at approximately 12-16 kDa in SDS-PAGE. It has a predicted molecular mass of 13.5 kDa. The protein separates into a number of isoforms with a pI between 9 and 10 in 2D PAGE due to post-translational modifications. The unmodified protein has a predicted pI of 9.
Purity >95%, as determined by SDS-PAGE and visualized by silver stain
Biol. activity The ED50 of beta NGF is typically 0.2-1.0 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line.
Form When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution It is recommended that 0.5 mL of sterile phosphate-buffered saline be added to the vial.
Storage Lyophilized products should be stored at 2 to 8C. Following reconstitution short-term storage at 2-8C is recommended, and longer-term storage of aliquots at -18 to -20C. Repeated freeze/thawing is not recommended.
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