||Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
||See product label
||Beta-nerve growth factor; beta-NGF; NGFB;
||Human - A DNA sequence encoding the human beta NGF protein sequence (containing the signal peptide, pro-peptide and the mature beta NGF sequence) was expressed in modified human 293 cells.
Theoretical peptide sequence:
||The NGF protein migrates at approximately 12-16 kDa in SDS-PAGE. It has a predicted molecular mass of 13.5 kDa. The protein separates into a number of isoforms with a pI between 9 and 10 in 2D PAGE due to post-translational modifications. The unmodified protein has a predicted pI of 9.
||>95%, as determined by SDS-PAGE and visualized by silver stain
||The ED50 of beta NGF is typically 0.2-1.0 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line.
||When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
||It is recommended that 0.5 mL of sterile phosphate-buffered saline be added to the vial.
||Lyophilized products should be stored at 2 to 8C. Following reconstitution short-term storage at 2-8C is recommended, and longer-term storage of aliquots at -18 to -20C. Repeated freeze/thawing is not recommended.