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Human Hepatocyte Growth Factor (HGF) protein expressed in mammalian cells

$297.00USD


Catalogue No. PE-1241-5
Description Hepatocyte growth factor (HGF) is a member of the plasminogen related growth factor family. The HGF protein is a dimer formed from an alpha and beta subunit linked by a disulfide bond. Both subunits originate from the endoproteolytic cleavage of a common precursor which is biologically inactive. HGF is expressed in Kupffer cella and sinusoidal endothelial cells in the liver and pancreas.
Batch No. See product label
Unit size 5 g
Other Names HGF; HPTA; Scatter factor; SF; Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin-A;
Accession P14210 HGF_HUMAN;
Produced in Human - A DNA sequence encoding the complete human Hepatocyte Growth Factor protein isoform 1 (comprising the signal peptide, alpha and beta chains) was expressed in modified human 293 cells.
Molecular Weight Under reducing conditions, the HGF protein migrates as a broad band between 80 and 110 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. The unmodified HGF has a predicted monomeric molecular mass of 79.7 kDa. The protein separates into a number of isoforms with a pI between 6 and 9 in 2D PAGE. The unmodified HGF protein has a predicted pI of 8.2
Purity >95%, as determined by SDS-PAGE and visualised by Coomassie Brilliant Blue
Biol. activity The ED50 of HGF is typically 20 - 40 ng/ml as measured in a cell proliferation assay using the human HepG2 cell line.
Form The HGF formulation consists of 0-30% carbohydrate by weight. When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage Lyophilised products should be stored at 2 to 8C. Following reconstitution short-term storage at 4C is recommended, and longer-term storage of aliquots at -18 to -20C. Repeated freeze thawing is not recommended.
Images (click to zoom)
Human Hepatocyte Growth Factor (HGF) protein expressed in mammalian cells Gel analysis of human HGF protein (PE-1241-5):

Lane 1 - HGF protein (5 µg)
Lane 2 - HGF protein (5 µg) treated with PNGase F
Lane 3 - HGF protein (5 µg) treated with glycosidase cocktail

Gel stained with Coomassie.

PDF Data Sheet

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MSDS

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