||Hepatocyte growth factor (HGF) is a member of the plasminogen related growth factor family. The HGF protein is a dimer formed from an alpha and beta subunit linked by a disulfide bond. Both subunits originate from the endoproteolytic cleavage of a common precursor which is biologically inactive. HGF is expressed in Kupffer cella and sinusoidal endothelial cells in the liver and pancreas.
||See product label
||HGF; HPTA; Scatter factor; SF; Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin-A;
||Human - A DNA sequence encoding the complete human Hepatocyte Growth Factor protein isoform 1 (comprising the signal peptide, alpha and beta chains) was expressed in modified human 293 cells.
||Under reducing conditions, the HGF protein migrates as a broad band between 80 and 110 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation. The unmodified HGF has a predicted monomeric molecular mass of
The protein separates into a number of isoforms with a pI between 6 and 9 in 2D PAGE. The unmodified HGF protein has a predicted pI of 8.2
||>95%, as determined by SDS-PAGE and visualised by Coomassie Brilliant Blue
||The ED50 of HGF is typically 20 - 40 ng/ml as measured in a cell proliferation assay
using the human HepG2 cell line.
||The HGF formulation consists of 0-30% carbohydrate by weight. When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
||It is recommended that 0.5 mL of sterile phosphate-buffered saline be added to the vial.
||Lyophilised products should be stored at 2 to 8C. Following reconstitution short-term storage at 2-8C is recommended, and longer-term storage of aliquots at -18 to -20C. Repeated freeze/thawing is not recommended.