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Human KLK8/Kallikrein ELISA Kit (1 Plate)

$497.00USD


Catalogue No. BEK-2366-1P
Description The human KLK8 kit is a sandwich ELISA. The capture antibody is a mouse monoclonal KLK8 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known KLK8 concentration are added to these wells and allowed to complex with the bound TREM2 antibody. A biotinylated goat polyclonal KLK8 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of KLK8 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human KLK8 in samples such as sera, plasma (heparin, EDTA), cell culture supernates and cell lysates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Unit size 1 plate
Antigen Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury (Ref: uniprot.org)
Other Names Neuropsin; NP; Ovasin; Serine protease 19; Serine protease TADG-14; Tumor-associated differentially expressed gene 14 protein
Accession O60259 KLK8_HUMAN
Produced in The standard for this kit is recombinant KLK8 protein produced in and purified from a NSO expression system using immunogen sequence Q29-G260.
Applications ELISA. The purpose of this kit is the in-vitro quantitative determination of human KLK8 in samples such as sera, plasma (heparin, EDTA), cell culture supernates and cell lysates.
Specificity Human
Cross-reactivity Does not cross-react with other relevant proteins
Storage Store at 2-8C
Expiry Date 6 months after purchase if unopened
Kit components The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Range 156 - 10,000 pg/mL
Sensitivity < 10 pg/mL
Kit protocol Click to download the kit protocolBEK-2366-1P_as_at_Jan2020_protocol.pdf
Images (click to zoom)
Human KLK8/Kallikrein ELISA Kit (1 Plate) This standard curve is for demonstration purposes only. A standard curve should be generated for each assay.

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

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