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Human Stem Cell Factor protein expressed in mammalian cells

$217.00USD


Catalogue No. PE-1242-5
Description Stem Cell Factor (SCF) is a hematopoietic growth factor that stimulates the proliferation of mast cells. SCF also mediates cell-cell adhesion and acts synergistically with other cytokines. The actions of SCF are exerted by binding to the SCF Receptor (PE-1243-50) which results in receptor dimerization. SCF is a type I membrane glycoprotein with 5 potential N-linked glycosylation sites. Soluble SCF is produced by proteolytic cleavage of the extracellular domain.
Batch No. See product label
Unit size 5 g
Other Names Kit ligand; C-kit ligand; Stem cell factor; SCF; Mast cell growth factor; MGF; KITLG; MGF; SCF;
Accession P21583 SCF_HUMAN;
Produced in Human - A DNA sequence encoding the signal peptide and the mature Stem Cell Factor (SCF) protein of human SCF was expressed in modified human 293 cells.
Molecular Weight SCF migrates as a broad band between 20 and 42 kDa in SDS-PAGE due to post-translational modifications. Unmodified SCF has a predicted molecular mass of 21.0 kDa. SCF separates into a number of isoforms with a pI between 3.0 and 7.0 in 2D PAGE due to post-translational modifications. This compares with the unmodified SCF with a predicted pI of 5.76.
Purity >95%, as determined by SDS-PAGE and visualized by silver stain
Biol. activity The ED50 of SCF is typically 10-20 ng/ml as measured in a cell proliferation assay using the human growth factor-dependent M-07e cell line.
Form Purified SCF consists of 0-50% carbohydrate by weight. When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage Lyophilized products should be stored at 2-8C. Following reconstitution, short-term storage at 4C is recommended and longer-term storage of aliquots at -18 to -20C. Repeated freeze thawing is not recommended.
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MSDS

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