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Mouse anti-Microtubule Associated Protein 2 (MAP2) [5H11]: IgG

$297.00USD


Catalogue No. M-1625-100
Description Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one 'a' subunit and one 'b' tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2 a and MAP2 b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
Batch No. See product label
Unit size 100l
Antigen High molecular MAP protein preparation derived from bovine brain
Clone 5H11
Other Names Microtubule-associated protein 2; MAP-2; Mtap2;
Accession Q0IIA8 MAP2_Bovine
Produced in Mouse
Molecular Weight http://http://www.uniprot.org/uniprot/Q0IIA8
Purity IgG
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A dilution of 1:5,000-1:10,000 is recommended for WB. A dilution of 1:500-1:1000 is recommended for IHC. The optimal dilution should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human; Rat; Mouse;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilized from PBS.
Reconstitution Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase.
Images (click to zoom)
Mouse anti-Microtubule Associated Protein 2 (MAP2) [5H11]: IgG Whole rat brain lysate with mouse anti-MAP2 antibody. The antibody recognizes the ~280 kDa protein. Right: Mixed neuron/glia cultures stained with mouse anti-MAP2 (green) and also rabbit antibody to neurofilament H (Catalog Number R-1388-50) (red). Since the NF-H protein is largely expressed in neuronal axons, while the MAP2 is only found in neuronal dendrites and perikarya, there is little overlap between these two staining patterns. DNA stain shows nuclei of neurons and non-neuronal cells (blue).


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MSDS

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