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Mouse antibody to human parvalbumin [3C9]: affinity purified

$297.00USD


Catalogue No. M-1813-100
Description In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. Ref: uniprot.org
Batch No. See product label.
Unit size 100 ug
Antigen Full-length recombinant human protein
Isotype IgG1
Clone 3C9
Accession P20472 (PRVA_HUMAN)
Produced in Mouse
Purity Affinity purified
Applications Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Note that this antibody does not recognize parvalbumin in rat or mouse brain homogenates on western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Reacts with human, rat, mouse. Antibody is specific for parvalbumin and does not recognize closely related proteins calretinin and calbindin as determined by Western Blotting.
Form Lyophilised from a solution containing PBS buffer pH 7.2-7.6 with 5 mM sodium azide as preservative.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile water. Centrifuge to remove any insoluble material. Final buffer contains preservatives.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Mouse antibody to human parvalbumin [3C9]: affinity purified Left: Staining of parvalbumin in adult rat cerebellum floating section by Immunohistochemistry. Mouse antibody to parvalbumin (1:1,000, green) stains dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue: Nuclear DNA stain. Right: Western blot analysis of parvalbumin expression in rat skeletal muscle homogenate (Lane 1). The antibody (1:1,000) detects parvalbumin at ~12 kDa in homogenate (Lane 1) and His-tagged parvalbumin protein (Lane 2) at slightly higher molecular weight. The antibody does not cross-react with His-tagged proteins calretinin (Lane 3) and calbindin (Lane 4). Note that this antibody does not recognize parvalbumin in rat or mouse brain lysates on western blots.
Mouse antibody to human parvalbumin [3C9]: affinity purified Rat cerebellum section stained with mouse anti-parvalbumin (green, 1:1,000) and chicken anti-calbindin (C-1798-50, red, 1:2,000) by Immunohistochemistry. Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 um, and free-floating sections were stained. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone, and so appear green.
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MSDS

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