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Mouse monoclonal antibody to beta Actin [AC-15]: IgG

$297.00USD


Catalogue No. M-1613-100
Description Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Beta actins coexist with gamma actins in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Batch No. See product label
Unit size 100 g
Antigen A modified synthetic peptide (C-DDDIAALVIDNGSGK) corresponding to the N-terminus of beta Actin conjugated to KLH.
Other Names ACTB; AX; actin; Actx;
Accession P60709 ACTB_HUMAN
Produced in Mouse
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25-0.5 g/ml is recommended for WB. Human beta actin has a predicted length of 375 residues and the MW of the monomer is 42 kDa. A concentration of 0.5-1.0 g/ml is recommended to detect beta actin in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Cross-reactivity Human; mouse; rat;
Form Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3
Reconstitution Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 g/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase.
PDF Data Sheet

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MSDS

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