||Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Beta actins coexist with gamma actins in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
||See product label
||A modified synthetic peptide (C-DDDIAALVIDNGSGK) corresponding to the N-terminus of beta Actin conjugated to KLH.
||ACTB; AX; actin; Actx;
||Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25-0.5 µg/ml is recommended for WB. Human beta actin has a predicted length of 375 residues and the MW of the monomer is 42 kDa. A concentration of 0.5-1.0 µg/ml is recommended to detect beta actin in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||Human; mouse; rat;
||Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3
||Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material.
||At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
||12 months after purchase.