||P Glycoprotein belongs to the MDR/TAP subfamily of ATP-binding cassette (ABC) transporters. P Glycoprotein is an ATP-dependent drug efflux pump expressed in liver, kidney, small intestine and brain. It is responsible for decreased drug accumulation in multidrug-resistant cells. Genetic variation in P Glycoprotein is thought to play a role in patients who do not respond to drug treatment. P Glycoprotein also functions as a transporter in the blood-brain barrier.
||See product label
||Human and hamster drug-resistant whole cells and crude plasma membranes
||Multidrug resistance protein 1; ATP-binding cassette sub-family B member 1; P-glycoprotein 1; CD243; ABCB1; MDR1; PGY1;
||Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/ml is recommended for WB. Human P Glycoprotein has a predicted length of 1280 residues and MW of 141 kDa. A concentration of 1.0-2.0 µg/ml is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 1.0-2.0 µg/ml is recommended for IHC to detect the protein in formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||The specificity of this antibody has been confirmed by WB and IHC against the antigen.
||If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
||Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3
||Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material.
||At least 12 months after purchase at 2 - 4°C (lyophilized formulations).
After reconstitution, aliquot and store at -20°C for a higher stability.
Avoid freeze-thaw cycles.
||12 months after purchase