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Mouse monoclonal antibody to human LPP3 [7H7D3]: Affinity purified

$297.00USD


Catalogue No. M-1560-100
Description Lipid phosphate phosphohydrolase 3 (LPP3) is a member of the phosphatidic acid phosphatase (PAP) family. LPP3 catalyzes the conversion of phosphatidic acid to diacylglycerol. In addition it hydrolyzes lysophosphatidic acid, ceramide-1-phosphate and sphingosine-1-phosphate (Ref: SWISSPROT).
Batch No. See product label.
Unit size 100 ug
Antigen A synthetic peptide from human LPP3 (179-196 aa) conjugated to KLH.
Sequence YRCRGDDSKVQEARKSFFc-KLH
Isotype Tests as a mouse IgG, with mixed subclasses of IgG1, 2a & 2b
Clone 7H7D3
Other Names Lipid phosphate phosphohydrolase 3; PAP2-beta; Phosphatidate phosphohydrolase type 2b; Phosphatidic acid phosphatase 2b; PAP-2b; PAP2b; Vascular endothelial growth factor and type I collagen-inducible protein; VCIP; PPAP2B;LPP3
Accession O14495 LPP3_HUMAN;
Produced in Mouse
Molecular Weight The LPP3 protein has a predicted length of 311 residues and the MW of the monomer is 35 kDa.
Applications Western Blotting (WB), Flow cytometry (FACS) and Immunohistochemistry (IHC). For WB, the recommended concentration is 2-3 ug/mL. For IHC, this antibody has been shown to work on formalin-fixed, paraffin-embedded tissue samples with heat-induced antigen retrieval. The recommended concentration is 0.5-2 ug/mL. For FACS, the recommended concentration is 2.0 ug/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Confirmed by over-expression of human LPP3 cDNA.
Cross-reactivity Human
Blast URL Click here
Form Lyophilised from PBS pH 7.4
Reconstitution Reconstitute in 100 uL of PBS pH 7.4. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2-8C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability and at 2-8C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Specific References Mueller PA (2016) PPAP2B expression limits lesion formation in murine models of atherosclerosis. Doctoral Dissertation. Species: Mouse. Application: IHC.

Reschen ME, Gaulton KJ, Lin D, Soilleux EJ, Morris AJ, Smyth SS and O'Callaghan CA (2015) Lipid-induced epigenomic changes in human macrophages identify a coronary artery disease-associated variant that regulates PPAP2B Expression through Altered C/EBP-beta binding. PLoS Genet. 2015 Apr 2;11(4):e1005061. Species: Human. Application: IHC with heat-induced antigen retrieval.

Humtsoe JO, Liu M, Malik AB and Wary KK (2010) Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Mol Cell Biol. Apr;30(7):1593-606. Species: Human. Application: WB and IHC.
PDF Data Sheet

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MSDS

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