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Mouse monoclonal antibody to human Musashi 1: IgG

$297.00USD


Catalogue No. M-1720-100
Description
Musashi 1 is an RNA binding protein that regulates the expression of target mRNAs at the translational level. It contains two conserved tandem RNA recognition motifs. Musashi 1 binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. It is detected in fetal kidney, brain, liver and lung and adult brain and pancreas. It is also detected in hepatoma cell lines.
Batch No. See product label
Unit size 100 ug
Antigen Full length recombinant human Musashi 1 protein (1-87) with a GST tag.
Isotype IgG2a
Other Names RNA-binding protein Musashi homolog 1; Musashi1; MSI1
Accession MSI1H_HUMAN
Produced in Mouse
Purity Protein G purified immunoglobulin
Applications This antibody is recommended for WB, IHC, sandwich ELISA and immunofluorescence. The recommended dilution for this antibody is 3 g/ml for IHC and 10 g/ml for immunofluorescence. The antibody has been used successfully on formalin fixed, paraffin embedded tissue. It is necessary to treat the paraffin sections by placing them in 1X Citrate Buffer (pH 6.0) then placing in a Pressure Cooker at 125degC for 4 minutes then at 90degC for 45 min. The tissue may also be treated by placing it in 1X Citrate Buffer (pH 6.0) then microwaving at 750W for 20 minutes. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specificity has been confirmed by WB and direct ELISA against the antigen.
Species Against Human
Blast it To see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence that you are interested in and copy and paste it HERE and blast/format it.
Form Lyophilised from PBS pH 7.4.
Reconstitution Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for up to six months. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date Lyophilized: 12 months after purchase
Images (click to zoom)
Mouse monoclonal antibody to human Musashi 1: IgG Western blot detection of Musashi 1 using the immunogen protein.

PDF Data Sheet

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MSDS

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