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Mouse monoclonal antibody to human NeuroD1 [3H8]: IgG

$297.00USD


Catalogue No. M-850-100
Description NeuroD1 is a differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. It is a transcriptional activator and binds to the insulin gene E-box. Efficient DNA binding requires dimerisation with another basic helix-loop-helix protein. In islet cells, NeuroD1 is phosphoryated on Ser-274 upon glucose stimulation and in activated neurons it is phosphorylated on Ser-335, which promotes dendritic growth. Defects in NeuroD1 cause maturity onset diabetes of the young type 6 (MODY6). It is characterised by the onset of diabetes during young adulthood and a primary defect in insulin secretion.
Batch No. See product label
Unit size 100 ug
Antigen Partial recombinant human NeuroD1 (201-300) with a GST tag.
Isotype IgG2a
Clone 3H8
Other Names Neurogenic differentiation factor 1; NeuroD; NEUROD1; NEUROD
Accession NDF1_HUMAN
Produced in Mouse
Purity Protein G purified immunoglobulin
Applications This antibody is recommended for WB, IHC, sandwich ELISA, immunofluorescence, Flow Cytometry. The recommended dilution for this antibody is 3 ug/mL for IHC and 10 ug/mL for immunofluorescence. Use ~2 ug per 10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specificity has been confirmed by WB and direct ELISA against the antigen.
Cross-reactivity Human. Other species have not been tested.
Blast it To see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence that you are interested in and copy and paste it HERE and blast/format it.
Form Lyophilised from PBS pH 7.2
Reconstitution Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for higher stability or at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse monoclonal antibody to human NeuroD1 [3H8]: IgG
A: Specific staining of NeuroD1 expressed in human neuroblastoma SH-SY5Y cell line by Flow Cytometry using cat # M-850-100. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 ug/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to NeuroD1 (2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 dilution), 20 minutes in dark at room temperature. Negative control: Non-specific Control IgG, clone X63 (cat # M-1249-200, black dashed). Data and results were generated using Orflo MoxiflowTM instrument and protocols. B: Immunohistochemical detection of NeuroD1 on formalin fixed, paraffin-embedded, human ovary, clear cell carcinoma. Anti-NeuroD1 primary antibody was used at a concentration of 3 µg/ml. C: Western blot detection of NeuroD1 expression in human neuroblastoma cell lysate. D: Immunofluorescent detection of NeuroD1 in HeLa cells. Antibody concentration: 10 µg/ml.

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MSDS

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