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Mouse monoclonal antibody to Microtubule-associated protein tau


Catalogue No. M-1703-100
Description FUNCTION: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. SUBCELLULAR LOCATION: Cytoplasm; cytosol. Cell membrane. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. ALTERNATIVE PRODUCTS: 8 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Isoforms differ from each other by the presence or absence of up to 5 of the 15 exons. One of these optional exons contains the additional tau/MAP repeat. TISSUE SPECIFICITY: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system. DEVELOPMENTAL STAGE: Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain. DOMAIN: The tau/MAP repeat binds to tubulin. In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments and straight filaments, mainly composed of hyperphosphorylated forms of Microtubule-associated protein Tau. Defects in Microtubule-associated protein Tau are a cause of frontotemporal dementia and parkinsonism linked to chromosome 17, as well as a number of other neurodegenerative diseases.
Batch No. See vial label
Unit size 100 uL
Antigen Recombinant full length version of the shortest human tau isoform purified from E. coli.
Antibody Type Monoclonal
Isotype IgG1
Other Names Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; MAPT; MTBT1; TAU
Produced in Mouse
Applications Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The antibody reacts with multiple closely spaced bands covering the region of the blot from 48kDa to 67kDa. It has also been used successfully for immunocytochemistry.
Species Against Human, rat and mouse. It is expected that it will work on other mammal tissues.
Form Lyophilised purified culture supernatant with 5% trehalose and 0.5% sodium azide.
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage Maintain lyophilized material at 2-8C. After reconstitution of lyophilized antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
General References 1. Skene JH, Willard M. Changes in axonally transported proteins during axon regeneration in toad retinal ganglion cells. J. Cell Biol. 89:86-95 (1981).
Images (click to zoom)
Mouse monoclonal antibody to Microtubule-associated protein tau Crude rat brain extract. Tau protein is expressed as up to 9 different isoforms of different molecular weight and so appears as multiple closely spaced bands covering the region of the blot from 48kDa to 67kDa.
Mouse monoclonal antibody to Microtubule-associated protein tau E18 hippocampal neurons were grown for seven days. The neurons were fixed and immunostained with M-1703-100 (red channel). The cells were also stained in green with C-1377-50, our chicken antibody to alpha Internexin and DNA (blue). M-1703-100 stains the neuronal perikarya and process strongly, and does not stain non neuronal cells in these cultures. The alpha internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.
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