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Mouse monoclonal antibody to Neurofilament Medium [3H11]


Catalogue No. M-1394-100
Description Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Batch No. See product label
Unit size 100 uL
Antigen Raised against a recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli. The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species.
Antibody Type Monoclonal
Isotype IgG1
Clone 3H11
Other Names Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Accession P12839 NFM_RAT;
Produced in Mouse
Applications Western Blotting (WB), Immunocytochemistry (IC), Immunohistochemistry (IH) and Flow Cytometry. A dilution of 1:2,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specifically recognizes the medium neurofilament subunit NF-L in WB.
Antibody Against Neurofilament Medium
Cross-reactivity Hu, Rat, Ms, Fel, Bov, Por, Chk
Blast URL Click here
Form Lyophilised with 5% trehalose
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Specific References 1. Felitsyn N. et al (2008) The heme precursor delta-aminolevulinate blocks peripheral myelin formation. J Neurochem. 2008 Sep;106(5):2068-79.
Images (click to zoom)
Mouse monoclonal antibody to Neurofilament Medium [3H11] Left: Culture of adult neural cells. Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H. The surrounding stellate red cells are stained with Rabbit polyclonal antibody to Internexin alpha R-1379-50. These are apparently mitotic neuronal progenitor cells and express many other neuronal markers. Right: Flow cytometry analysis of endogenously expressed Neurofilament Medium on mouse neural progenitor cells differentiated from mES and fixed overnight in 70% ethanol (Red curve). PE-labelled goat anti-mouse IgG was used as secondary antibody (Blue curve).  Negative control processed with secondary antibody only. Data was acquired on Moxi FlowTM.
Mouse monoclonal antibody to Neurofilament Medium [3H11] Left: Analysis of Neurofilament Medium expression in rat frontal cortex section by Immunohistochemistry. Section was stained with mouse antibody to NF-M (green, 1:5,000), and co-stained with chicken antibody to neurofilament heavy (C-1386-50, red, 1:5,000). IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 um sections, and free-floating sections were stained. The NF-M mouse antibody labels neuronal cell bodies and dendrites of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the chicken NF-H antibody stains the network of neuronal axons only. Right: Western blot analysis of neuronal tissue lysates for NF-M expression (green, 1:10,000): [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] cow spinal cord, [5] rat sciatic nerve. Strong bands at 145 kDa correspond to rodent NF-M, while the bovine NF-M protein appears larger and migrates at about 160 kDa.
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