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Mouse monoclonal antibody to Neurofilament Medium [3H11]


Catalogue No. M-1394-100
Description Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Batch No. See product label
Unit size 100 uL
Antigen Raised against a recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli. The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species.
Antibody Type Monoclonal
Isotype IgG1
Clone 3H11
Other Names Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Accession P12839 NFM_RAT;
Produced in Mouse
Applications Western Blotting (WB), Immunocytochemistry (IC), Immunohistochemistry (IH) and Flow Cytometry. A dilution of 1:1,000 - 1:5,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specifically recognizes the medium neurofilament subunit NF-L in WB.
Antibody Against Neurofilament Medium
Cross-reactivity Hu, Rat, Ms, Fel, Bov, Por, Chk
Blast URL Click here
Form Lyophilised with 5% trehalose
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Specific References 1. Felitsyn N. et al (2008) The heme precursor delta-aminolevulinate blocks peripheral myelin formation. J Neurochem. 2008 Sep;106(5):2068-79.
Images (click to zoom)
Mouse monoclonal antibody to Neurofilament Medium [3H11] Left: Culture of adult neural cells. Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H. The surrounding stellate red cells are stained with Rabbit polyclonal antibody to Internexin alpha R-1379-50. These are apparently mitotic neuronal progenitor cells and express many other neuronal markers. Right: Flow cytometry analysis of endogenously expressed Neurofilament Medium on mouse neural progenitor cells differentiated from mES and fixed overnight in 70% ethanol (Red curve). PE-labelled goat anti-mouse IgG was used as secondary antibody (Blue curve).  Negative control processed with secondary antibody only. Data was acquired on Moxi FlowTM.

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