||CD4 is a single-pass type I membrane protein expressed on the surface of T helper/inducer cells, regulatory T cells, monocytes, macrophages, dendritic cells and microglia.
||See product label
||CD4 transfected mouse T-cell hybridoma 3DT followed by CD4+ human T-cell CEM cell line
||T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; CD4mut;
||P01730 CD4_HUMAN; P05540 CD4_RAT;
||Immunohistochemistry (IHC-Frozen). A concentration of 1.0 µg/ml is recommended to detect CD4 in formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||The specificity of this antibody has been confirmed by immunohistochemistry against the antigen.
||If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practice is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
||Liquid (0.5ml). 50% glycerol, 0.9mg NaCl and 0.2mg Na2HPO4
||The liquid formulation should be diluted in PBS (pH 7.4)
||After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
||12 months after purchase
||1. Almolda B. et al. CD4 microglial expression correlates with spontaneous clinical improvement in the acute Lewis rat EAE model. J Neuroimmunol. 2009 Apr 30;209(1-2):65-80.
2. Jordan C.A. et al. Infection of brain microglial cells by human immunodeficiency virus type 1 is CD4 dependent. J Virol. 1991 Feb;65(2):736-42.