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Mouse monoclonal antibody to proBDNF (47-58), [Clone BS375]: IgG


Catalogue No. M-1729-100
Description Brain derived neurotrophic factor (BDNF) is synthesized as a precursor (proBDNF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proBDNF is synthesized in neurons and glia (eg., microglia), transported anterogradely and retrogradely and may be released in an activity dependent manner.
Unit size 100 ug
Antigen A synthetic peptide (C-NGPKAGSRGLTS, aa: 47-58) of human proBDNF protein has been used as the immunogen. The sequence is located on the pro-domain of the proBDNF full-length protein.
Isotype IgG1, kappa
Other Names Abrineurin
Accession BDNF_HUMAN
Produced in Mouse
Purity Protein G purified mouse IgG
Applications Flow Cytometry (2 ug/106 cells).

Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Human
Cross-reactivity Not yet tested. Antibody may detect mouse and rat proBDNF protein due to high degree of amino acid sequence homology.
Form Lyophilised from a solution containing PBS buffer pH 7.4 with 3% trehalose, without preservatives.
Appearance Dry powder.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage After reconstitution divide into aliquots and store at -20C for a higher stability. Antibody contains no preservatives. Store at 2-8C with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Mouse monoclonal antibody to proBDNF (47-58), [Clone BS375]: IgG Detection of proBDNF expression in human neuroblastoma cell line SHSY-5Y by Flow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 ug/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to proBDNF (cat # M-1729-100, 2 μg per ~106 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.

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