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Mouse monoclonal antibody to proGDNF (55-66), [Clone BS376]: IgG

$297.00USD


Catalogue No. M-1727-100
Description Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (Ref: uniprot.org). ProGDNF is the unprocessed precursor molecule of mature GDNF and exists as homodimer.
Batch No. See product label.
Unit size 100 g
Antigen A synthetic peptide (C-EDYPDQFDDVMD, aa: 55-66) of human proGDNF protein has been used as the immunogen. The sequence is located on the pro-domain of the proGDNF full-length protein and is homologous with mouse and rat form of proGDNF.
Antibody Type Mouse monoclonal IgG antibody
Isotype IgG3, kappa
Clone BS376
Other Names Glial cell line-derived neurotrophic factor; Astrocyte-derived trophic factor 1; ATF-1
Accession GDNF_HUMAN
GDNF_MOUSE
GDNF_RAT
Produced in Mouse
Purity Protein G purified mouse IgG
Applications Flow Cytometry (2 g/ 106 cells). Immunocytochemistry (1-2 g/mL). Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Human
Cross-reactivity Rat. Antibody is expected to detect mouse proGDNF protein due to 100% amino acid sequence homology.
Form Lyophilised from a solution containing PBS buffer pH 7.4 with 3% trehalose, without preservatives.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 L of sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage After reconstitution divide into aliquots and store at -20C for a higher stability. Antibody contains no preservatives. Store at 4C with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened
Images (click to zoom)
Mouse monoclonal antibody to proGDNF (55-66), [Clone BS376]: IgG Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 1% BSA, Primary antibody: Mouse Monoclonal antibody to proGDNF (cat # M-1727-100, 2 μg per ~106 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols. The data demonstrates specific staining of proGDNF expressed in human neuronal cell line SHSY-5Y using cat # M-1727-100.

Mouse monoclonal antibody to proGDNF (55-66), [Clone BS376]: IgG Immunofluorescence analysis of proGDNF expression in human SH-SY5Y cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X100) SH-SY5Y cells were incubated with proGDNF antibody M-1727-100 (2 μg/mL, green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). proGDNF-IR is observed throughout the cytoplasm. Magnification: 100x.
Mouse monoclonal antibody to proGDNF (55-66), [Clone BS376]: IgG Immunofluorescence analysis of proGDNF expression in rat C6 glioma cells. Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X100) C6 cells were incubated with proGDNF antibody M-1727-100 (2 μg/mL, green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). proNGF-IR is observed in the perinuclear cytoplasm. Magnification: 100x.
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