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Mouse monoclonal antibody to Proliferating Cell Nuclear Antigen (PCNA) [IML-83]: IgG

$297.00USD


Catalogue No. M-990-100
Description Proliferating cell nuclear antigen (PCNA) belongs to the DNA sliding clamp family. PCNA helps increase the processivity of DNA polymerase during leading strand synthesis in DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway.
Batch No. See product label
Unit size 100 g
Antigen Protein A-PCNA fusion obtained from rat liver tissue.
Clone IML-83
Other Names PCNA; Cyclin;
Accession P12004 PCNA_HUMAN; P04961 PCNA_RAT
Produced in Mouse
Purity IgG
Applications Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 g/ml is recommended for WB. Human PCNA has a predicted length of 261 residues and MW of 29 kDa. A concentration of 0.4-1.0 g/ml is recommended to detect PCNA in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Antigen retrieval may improve staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Cross-reactivity Human; mouse; rat
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3
Reconstitution Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 g/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4C (lyophilized formulations). After reconstitution, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse monoclonal antibody to Proliferating Cell Nuclear Antigen (PCNA) [IML-83]:  IgG Immunohistochemical detection of PCNA in paraffin embedded HELA cell sections using Mouse monoclonal antibody to PCNA (M-990-100) at 0.4-1.0 µg/ml. Secondary antibody was biotinylated goat anti-mouse at a concentration of 10.0 µg/ml.
Mouse monoclonal antibody to Proliferating Cell Nuclear Antigen (PCNA) [IML-83]:  IgG Western blot of PCNA antibody M-990-100 at 2 μg/ml in MCF7 whole cell lysate (lane 1), Hela whole cell lysate (lane 2), HT1080 whole cell lysate (lane 3) and Colo320 whole cell lysate (lane 4). Secondary antibody HRP conjugated goat anti-mouse IgG at 1:3000-10000.
PDF Data Sheet

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MSDS

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