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||See product label
||Bovine kidney S100 beta
||S100B; Calcium binding protein B1;
|| P02638 S100B_BOVIN
||Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 µg/ml is recommended for WB. The predicted length of bovine S100 beta subunit is 92 residues and the MW of the monomer is 11 kDa. A concentration of 1.0-2.0 µg/ml is recommended to detect S100 beta subunit in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||The specificity of this antibody has been confirmed by WB and IHC against the antigen.
||Human; mouse; rat
||If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
||Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3
||Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material.
||At least 12 months after purchase at 2 - 4°C (lyophilized formulations).
After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
||12 months after purchase.
||Yasuda Y. et al. Relationship between S100beta and GFAP expression in astrocytes during infarction and glial scar formation after mild transient ischemia. Brain Res. 2004 Sep 17;1021(1):20-31.