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Mouse monoclonal antibody to splicing factor SF3B4 [3A1]: Affinity purified

$297.00USD


Catalogue No. M-1576-100
Description SF3B4 is one of 8 subunits of splicing factor SF3B. SF3B4 is ubiquitously expressed in the nuclei of eukaryotic cells, although it migrates into the cytoplasm of dividing cells.
Batch No. See product label
Unit size 100 g
Antigen Full length recombinant human SF3B4 which was expressed in and purified from E. coli.
Isotype IgG2b
Clone 3A1
Other Names SAP49; splicing factor 3b subunit 4; 49kDa SAP49; spliceosome-associated protein 49; U2 snRNP; Hsh49; MGC108282; SF3B4; SF3b50;
Accession Q15427 SF3B4_HUMAN;
Produced in Mouse
Applications WB, IHC/IF, Flow Cytometry. Recommended dilution of at least 1:100 for IHC/IF. In WB using chemiluminescence it can be used at dilutions of 1:1,000 or lower. The protein runs on SDS-PAGE gels at an apparent molecular weight of 49kDa. Use 2ug/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Cross-reactivity Human; bovine; porcine; mouse; rat; expected to react with other species due to sequence homology
Blast URL Click here
Form Lyophilised with 5% trehalose
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage Aliquot and store at -20C for a higher stability and at 4C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse monoclonal antibody to splicing factor SF3B4 [3A1]: Affinity purified Left: Human HeLa cells stained with Mouse monoclonal antibody to splicing factor SF3B4 M-1576-100 (red), Chicken polyclonal antibody to Vimentin C-1409-50 (green) and DNA (blue, stained with DAPI). The monoclonal SF3B4 antibody reveals strong granular nuclear staining which is a little different from the DNA stain and presumably reflects splicosomal complexes. The polyclonal Vimentin antibody stains the cytoplasmic intermediate filament network of the HeLa cells. Right: Analysis of SF3B4 expression in rat pheochromocytoma PC-12 cell line by Flow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 1% BSA, Primary antibody: Mouse Monoclonal antibody to SF3B4 (cat # M-1576-100, 2μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.

PDF Data Sheet

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MSDS

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