||Synaptophysin is a 38 kDa glycoprotein present in the membranes of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions and endocrine cells. It is also expressed by neuroendocrine cells, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms.
||See product label
||Rat retina synaptosome
||Syp; p38; SVP38; SP; SYN; SYP1;
||Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/ml is recommended for WB. Rat synaptophysin has a predicted length of 307 residues and a MW of 38 kDa. A concentration of 1.0-2.0 µg/ml is recommended to detect synaptophysin in formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
||The specificity of this antibody has been confirmed by WB and IHC against the antigen.
||If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.
The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please chose carefully, as the sequence you chose may be a precursor rather than the mature protein for example.
||Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3
||Reconstitute in 1 ml of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/ml. Centrifuge to remove any insoluble material.
||At least 12 months after purchase at 2 - 4°C (lyophilized formulations).
After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
||12 months after purchase