biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact us
 


Technical Notes
 
View our tech notes
 
 
Follow us...
 
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive
 
 

Mouse monoclonal antibody to Tyrosine Kinase Receptor A (TrkA, 235-246), extracellular domain [Clone BS470]: IgG

$297.00USD


Catalogue No. M-1723-100
Description TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer (Reference: www.uniprot.com).
Batch No. Please refer to the product label.
Unit size 100 g
Antigen A synthetic peptide from the extracellular domain of human TrkA (C-SATVMKSGGLPS, aa: 235-246) has been used as the immunogen.
Sequence SATVMKSGGLPS
Antigen Location Peptide sequence is located within the extracellular domain consisting of amino acids 33-432.
Antigen Length 12 amino acids
Antibody Type Mouse monoclonal IgG
Isotype IgG3, k-light chain
Clone BS470
Other Names Tropomyosin-related kinase receptor; High affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; TRK1 transforming tyrosine kinase protein; p140-TrkA; Trk-A; NTRK1; TRK
Accession Uniprot: P04629; NTRK1_HUMAN
Produced in Mouse
Molecular Weight Glycosylated human TrkA has an apparent molecular weight of 140 kDa
Purity Protein G purified mouse immunoglobulin
Applications Flow cytometry: 20 g/mL.

Immunocytochemistry: 1-5 g/mL.

Flow cytometry data and immunofluorescence staining of TrkA (extracellular domain) receptor in SHSY-5Y cells has shown that detection of TrkA expression depends on its cellular localization (membrane vs. internal stores). Thus, permeabilization of cells may be required, despite that clone BS470 was raised against the extracellular domain of TrkA.

Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity TrkA. Does not cross-react with TrkB or TrkC.
Species Against Human
Antibody Against Human
Cross-reactivity Reacts with human TrkA. While not tested yet, M-1723-100 is not expected to react with TrkA from other species due to amino acid sequence dissimilarity.
Form Lyophilized from PBS, pH 7.4, containing 3% trehalose without preservatives.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 L of sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage After reconstitution divide into aliquots and store at -20C for a higher stability. Antibody contains no preservatives. Store at 4C with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened.
Images (click to zoom)
Mouse monoclonal antibody to Tyrosine Kinase Receptor A (TrkA, 235-246), extracellular domain [Clone BS470]: IgG TrkA expression on non-permeabilized human SHSY-5Y neuroblastoma and DU145 prostate cancer cell lines analysed by Flow Cytometry. Blocking: 200 μg/mL normal sheep IgG (30 minutes) on ice. Primary antibody: Mouse Monoclonal antibody to TrkA, extracellular domain (cat # M-1723-100, 2 μg per ~106 cells) for 30 minutes on ice. Secondary antibody: Goat anti-mouse PE (1:100 dilution, 20 min in dark on ice. Non-specific Control IgG, clone X63 (cat# M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo Moxiflow™ instrument and protocols.
Mouse monoclonal antibody to Tyrosine Kinase Receptor A (TrkA, 235-246), extracellular domain [Clone BS470]: IgG Immunofluorescence analysis of TrkA expression on human SHSY-5Y neuroblastoma cell membrane. Fixed (4% formaldehyde), non-permeabilized, and blocked (10% normal horse serum, 0.1% Triton X100) SHSY-5Y cells were incubated with TrkA antibody M-1723-100 (extracellular domain, 2 μg/mL, green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). Magnification: 100x.
Mouse monoclonal antibody to Tyrosine Kinase Receptor A (TrkA, 235-246), extracellular domain [Clone BS470]: IgG Immunofluorescence analysis of TrkA expression in human SHSY-5Y neuroblastoma cells, comparing TrkA antibodies M-1723-100 (BS470, extracellular domain) and M-1719-100 (BS292, intracellular domain). Cells were fixed (4% formaldehyde, 10 minutes), permeabilized (0.1% Triton X100, A and C) or non-permeabilized (A), and blocked (10% normal horse serum) for 30 minutes. SHSY-5Y cells were incubated for 1 hour with TrkA primary antibodies (2 μg/mL, green) M-1723-100 (extracellular domain, A1 and B1) and M-1719-100 (intracellular domain, C1). Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). Control cells were treated exactly the same way, substituting the primary TrkA antibody with normal mouse IgG (A2, B2 and C2) and using the same camera exposure times to record images. Magnification: 100x. This batch of SHSY-5Y cells did not show TrkA expression on the cell membrane (A), while both antibodies were immunoreactive for intracellulary stored TrkA receptor (B and C).
Mouse monoclonal antibody to Tyrosine Kinase Receptor A (TrkA, 235-246), extracellular domain [Clone BS470]: IgG Comparison of TrkA expression on non-permeabilized and permeabilized human SHSY-5Y neuroblastoma cells by Flow Cytometry. This batch of SHSY-5Y cells did not show TrkA expression on the membrane (left image), but immunoreactivity in permeabilized cells (right image), suggesting that TrkA is stored intracellularly. Conditions: Permeabilization with absolute methanol (10 minutes on ice), or no permeabilization (incubation of primary antibody on ice only). Blocking: 200 μg/mL normal sheep IgG (30 minutes) on ice. Primary antibody: Mouse Monoclonal antibody to TrkA, extracellular domain (cat# M-1723-100, 2 μg per ~106 cells) for 30 minutes on ice. Secondary antibody: Goat anti-mouse PE (1:100 dilution, 20 min in dark on ice. Non-specific Control IgG, clone X63 (cat# M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo Moxiflow instrument and protocols.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
 
0 items
 
 
Currencies
 
  
 
 
Email This Page
 
 
Tell someone you know about this product.
 
 
We Accept PayPal
 
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment


Official PayPal Seal