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Mouse monoclonal antibody to Tyrosine Kinase Receptor B (TrkB, 179-190), extracellular domain [Clone BS379]: IgG

$297.00USD


Catalogue No. M-1836-100
Description The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
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R-1834-100, Rabbit antibody to Tyrosine Kinase Receptor B (TrkB; 70-90): affinity purified
Batch No. See product label
Unit size 100 ug
Antigen Synthetic peptide immunogen, ESSKNIPLANLQ
Sequence ESSKNIPLANLQ
Antigen Location Extracellular domain of TrkB (amino acids 179-190 of human TrkB)
Antibody Type Mouse monoclonal
Isotype IgG2b, kappa
Clone BS379
Other Names GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B
Accession NTRK2_HUMAN
Produced in Mouse
Purity Protein G purified
Applications Flow Cytometry (5-10 ug/mL): Tested on human and rodent cell lines. Cell staining can be performed under native conditions on ice, or on fixed cells with up to 4% formaldehyde.
Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity TrkB
Species Against Human
Cross-reactivity Mouse and rat TrkB. Species cross-reactivity determined by Flow Cytometry.
Form Lyophilized from PBS, pH 7.2-7.6 with 0.1% trehalose.
Reconstitution Spin vial briefly before opening. Reconstitute in 100 uL sterile distilled water to obtain an antibody concentration of 1 mg/mL. Centrifuge to remove any insoluble material.
Storage Store lyophilised antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened
Images (click to zoom)
Mouse monoclonal antibody to Tyrosine Kinase Receptor B (TrkB, 179-190), extracellular domain [Clone BS379]: IgG TrkB expression (red) in 50B11 mouse x rat hybrid cell line analysed by Flow Cytometry. Fixation: 0 ,2 and 4% formaldehyde in PBS, pH 7.2-7.6 on ice (10 minutes). Blocking: 100 ug/mL normal sheep IgG in PBS (30 minutes) on ice. Primary antibody: TrkB clone BS379 (10 ug/mL) for 60 minutes on ice. Secondary antibody: Goat anti-rabbit PE (1:100 dilution), 30 min in dark on ice. Non-specific control IgG clone X63 (black) was used as negative control under same conditions. Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Mouse monoclonal antibody to Tyrosine Kinase Receptor B (TrkB, 179-190), extracellular domain [Clone BS379]: IgG TrkB expression (red) in human SHSY5Y and HeLa cell lines analysed by Flow Cytometry. Staining was performed on unfixed cells on ice. Blocking: 100 ug/mL normal sheep IgG in PBS (30 minutes) on ice. Primary antibody: TrkB clone BS379 (10 ug/mL) for 60 minutes on ice. Secondary antibody: Goat anti-rabbit PE (1:100 dilution), 30 min in dark on ice. Non-specific control IgG clone X63 (black) was used as negative control under same conditions. Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
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MSDS

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