biosensis fine bioscience tools - antibodies, elisa kits, proteins, toxins and peptides An Australia-US partnershipPhone: 1800 605 5127 in the US, or +61 8 8352 7711 for international enquiries to Australia.
HomeAbout BiosensisBiosensis distributorsResourcesHelp deskordering from BiosensisContact usFlyers

Technical Notes
View our tech notes
Follow us...
Google+ Follow us on Twitter

Find us on Facebook

Sign up for Biosensis newsletters
Visit our newsletter archive

Mouse monoclonal antibody to Visinin-like protein 1 [2D11]: IgG


Catalogue No. M-1657-100
Description Visinin (sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1) was originally isolated biochemically from chicken retina as a major protein of about 24kDa on SDS-PAGE (1). Following cloning and sequencing of visinin, several visinin like proteins were discovered by homology screening (2, 3). One of these, Visinin-like protein 1 is a small Calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression (4, 5). It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein belongs to the large superfamly of calmodulin and paravalbumin type proteins which function by binding Calcium ions. Calcium binding alters the confomation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like protein 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides which are responsible for Calcium binding. Visinin-like protein 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of 22kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury (6, 7, 8).
Unit size 100 ug
Antigen Recombinant Visinin-like protein 1 expressed and purified from E. coli.
Antibody Type Monoclonal
Isotype IgG1
Clone 2D11
Other Names Hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1
Produced in Mouse
Applications Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:500 - 1:1,000 is recommended for WB. A dilution of 1:500-1:1,000 is recommended for IHC and ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The antibody reacts with a 22 kDa band by Western blot on bovine cerebellum lysate. It has also been used successfully for immunocytochemistry.
Species Against Human, bovine, rat and mouse. It is expected that it will work on other mammal tissues.
Antibody Against Visinin-like protein 1
Form Lyophilised from PBS. Contains 5% trehalose.
Appearance White powder
Reconstitution Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Storage After reconstitution of lyophilised antibody, aliquot and store at -20C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase
General References 1. Hatakenaka S, Kuo CH, Miki N. Analysis of a distinctive protein in chick retina during development. Brain Res. 312:155-63 (1983).
2. Kuno T, Kajimoto Y, Hashimoto T, Mukai H, Shirai Y, Saheki S, Tanaka C. cDNA cloning of a neural visinin-like Ca(2+)-binding protein. Biochem Biophys Res Commun. 184:1219-25 (1992).
3. Polymeropoulos MH, Ide S, Soares MB, Lennon GG. Sequence characterization and genetic mapping of the human VSNL1 gene, a homologue of the rat visinin-like peptide RNVP1. Genomics. 29:273-5 (1995).
4. Bernstein HG, Baumann B, Danos P, Diekmann S, Bogerts B, Gundelfinger ED, Braunewell KH. Regional and cellular distribution of neural visinin-like protein immunoreactivities (VILIP-1 and VILIP-3) in human brain. J Neurocytol. 28:655-62 (1999).
5. Paterlini M, Revilla V, Grant AL, Wisden W Expression of the neuronal calcium sensor protein family in the rat brain. .Neuroscience. 99:205-16 (2000)
6. Laterza OF, Modur VR, Crimmins DL, Olander JV, Landt Y, Lee JM, Ladenson JH. Identification of novel brain biomarkers. Clin. Chem. 9:1713-21 (2006)
7. Lee JM, Blennow K, Andreasen N, Laterza O, Modur V, Olander J, Gao F, Ohlendorf M, Ladenson JH. The brain injury biomarker VLP-1 is increased in the cerebrospinal fluid of Alzheimer disease patients. Clin. Chem. 10:1617-23 (2008).
8. Tarawneh R, D'Angelo G, Macy E, Xiong C, Carter D, Cairns NJ, Fagan AM, Head D, Mintun MA, Ladenson JH, Lee JM, Morris JC, Holtzman DM. Visinin-like protein-1: diagnostic and prognostic biomarker in Alzheimer disease. Ann Neurol. 70:274-85 (2011) doi: 10.1002/ana.22448.
Images (click to zoom)
Mouse monoclonal antibody to Visinin-like protein 1 [2D11]: IgG Western blot of bovine cerebellum homogenate stained with M-1657-100.
Mouse monoclonal antibody to Visinin-like protein 1 [2D11]: IgG Confocal image of adult rat cerebellar cortex stained with M-1657-100 (green), our chicken polyclonal antibody to MAP2 (Catalog Number C-1382-50, red) and DNA (blue). The M-1657-100 antibody reveals synapses in the molecular layer (ML) strongly. Synaptic regions are also seen in the granule cell layer (GC). The perikarya of Purkinje cells (PC) are revealed with MAP2 antibody (4). Little staining is seen in the white matter (WM).
Mouse monoclonal antibody to Visinin-like protein 1 [2D11]: IgG Left: VILIP-1 staining of rat cerebellum section by Immunohistochemistry. Section was stained with mouse antibody to VILIP-1 (red, 1:500) and rabbit antibody to GFAP (R-1374-50, green, 1:5,000). Blue: DAPI nuclear stain.IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The VILIP-1 antibody reveals protein expressed in granule cell membranes and in synapses in the white matter and molecular layers of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astroglia. Right: Western blot analysis of VILIP-1 expression (green, 1:1,000) in tissue lysates. [1] protein standard, [2] rat brain [3] rat cerebellum, and [4] mouse brain. The band at around 20 kDa corresponds to VILIP-1 protein.
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet


Click to download the MSDS - Click to download the MSDS

Offices in the USA and Australia
My Ice Bucket  
0 items
Email This Page
Tell someone you know about this product.
We Accept PayPal
You can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_IDYou can use secured services of paypal to send us money via our email account MODULE_PAYMENT_PAYPAL_IPN_ID
Click to make a payment

Official PayPal Seal