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Mouse nerve growth factor (2.5S) protein (NGF)


Catalogue No. PE-019-25
Description Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500 Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF (see reference below).
Batch No. See product label
Unit size 25 ug
Other Names mouse NGF; beta NGF
Accession NGF_MOUSE
Produced in Mouse
Molecular Weight The homodimer has a molecular weight of ~26KDa and the monomer ~13KDa as confirmed by SDS PAGE.
Purity Greater than 90% (as determined by SDS electrophoresis)
Biol. activity Tested by stimulation of 8 day old chick embryo dorsal root ganglia.
Form Lyophilised from PBS, pH 7.4 without preservatives.
Appearance White powder.
Reconstitution Reconstitute with the addition of 25 uL sterile water and divide into single use aliquots.
Storage Store lyophilized protein at 2-8C. After reconstitution, store at 2-8C short term. Store long-term at -20C to -80C. Avoid repeated freezing and thawing. See expiration date for shelf-life estimates, actual times may vary depending upon experimental conditions and laboratory handling.
Expiry Date 12 months upon purchase for dry material. Hydrated material, 5 days at 4C, 30 days -20C, 60 days -70C for highest activity Avoid repeated freezing and thawing. Use insulated storage containers for best results.
Specific References Carter DR et al (2016) Glutathione biosynthesis is upregulated at the initiation of MYCN-driven neuroblastoma tumorigenesis. Mol Oncol. 2016 Jun;10(6):866-78.

Goodhill GJ et al (2015) The dynamics of growth cone morphology. BMC Biol. 2015 Feb;13(10).

Coulson EJ et al (2015) Neurotrophin-tyrosine kinase receptor signaling. United States Patent Application 20150344535.

Gearing, DP et al (2013) A fully caninised anti-NGF monoclonal antibody for pain relief in dogs. BMC Vet Res. 9:226.

Matusica D et al (2013) An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function. J Biol Chem. 2013 Apr 19;288(16):11144-54.

Yuan J et al (2013) Optimality and saturation in axonal chemotaxis. Neural Comput. 2013 Apr;25(4):833-53.

Sykes AM et al (2012) The effects of transmembrane sequence and dimerization on cleavage of the p75 neurotrophin receptor by γ-secretase. J Biol Chem. 2012 Dec 21;287(52):43810-24.

E.M. Forbes et al (2012) Calcium and cAMP Levels Interact to Determine Attraction versus Repulsion in Axon Guidance. Neuron. 2012 May 10;74(3):490-503.
General References Mobley WC, Schenker A, Shooter EM (1976) Biochemistry. 15(25) pp. 5543-52.
Images (click to zoom)
Mouse nerve growth factor (2.5S) protein (NGF) NGF-induced differentiation of PC12 cells in culture. PC12 cells were plated on poly-lysine-coated coverslips and treated with 75 ng/mL native mouse NGF for 19 days. Cells start to differentiate after 6 days, forming a dense neurite network. Images were taken with a light microscope at 10X magnification.

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