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Mouse polyclonal antibody to human Semaphorin 6A: whole serum

$297.00USD


Catalogue No. Mo-952-50
Description The transmembrane semaphorin Semaphorin 6A is expressed in developing neural tissue and is required for proper development of the thalamocortical neuron projection. Semaphorin 6A belongs to a subfamily characterized by an extracellular semaphorin domain, a transmembrane domain, and a long cytoplasmic tail. Members of this class can repel sympathetic and dorsal root ganglion axons in vitro, consistent with a traditional role as guidance signals. It was also suggested that Semaphorin 6A has a role in retrograde signaling.
Batch No. See product label
Unit size 50 uL
Antigen Partial recombinant human Semaphorin 6A (21-120) with a GST tag.
Other Names Semaphorin VIA; Sema VIA; Semaphorin-6A-1; SEMA6A-1; SEMA6A; KIAA1368; SEMAQ
Accession SEM6A_HUMAN
Produced in Mouse
Purity Whole serum
Applications This antibody is recommended for WB, ELISA and Flow Cytometry. Use 1:100 fold dilution for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Specificity has been confirmed by WB and direct ELISA against the antigen.
Cross-reactivity Human. Other species have not been tested.
Blast it To see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence that you are interested in and copy and paste it HERE and blast/format it.
Form Liquid. 50% glycerol
Storage Keep aliquots at -20C for higher stability or at 2-8C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
Images (click to zoom)
Mouse polyclonal antibody to human Semaphorin 6A: whole serum Left: Western blot detection of GST tagged recombinant human  Semaphorin 6A. Note the GST tag alone is 26 kDa. Right: Analysis of SEMA6A expression in human neuroblastoma SH-SY5Y cell line by Flow Cytometry.Cells were washed twice using PBS + 0.1% NaN3, Blocking: 200 ug/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to SEMA6A (cat # Mo-952-100, 1:100 fold dilution for ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.

PDF Data Sheet

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MSDS

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