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Mouse Transforming growth factor beta-1 ELISA Kit (1 plate)

$398.50USD


Catalogue No. BEK-2095-1P
  Find a 2 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com
Description The mouse Transforming growth factor beta-1 (TGFb1) Kit is a sandwich ELISA. The capture antibody is a monoclonal TGFb1 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TGFb1 concentration are added to these wells and allowed to complex with the bound TGFb1 antibody. A biotinylated TGFb1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TGFb1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TGFb1 cell culture supernatants, serum, plasma(EDTA) and urine. Acid activation is required for best activity. Check that samples are neutralized prior to running the assay. Free TGFB1 is highly labile. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Batch No. See product labels
Other Names TGFB1; TGFB; TGF-beta-1;
Accession P04202 TGFB1_MOUSE;
Specificity Human; Mouse; Rat;
Cross-reactivity The cross reactivity with TGFβ2, TGFβ3 and TGFβ5 is < 1%.
Storage Store at 4øC
Kit components The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Range 15.6 pg/ml - 1,000 pg/ml
Sensitivity < 2 pg/ml
Kit protocol Click to download the kit protocolBEK-2095-1P_as_at_Jun2017_protocol.pdf
Images (click to zoom)
Mouse Transforming growth factor beta-1 ELISA Kit (1 plate) This standard curve is for demonstration purposes only. A standard curve should be generated for each assay.

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

MSDS

Click to download the MSDS - Click to download the MSDS

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