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Multi-Neurotrophin Rapid Screening ELISA Kit: Mouse


Catalogue No. BEK-2231
Description The biosensis? Multi-Neurotrophin RapidTM Screening ELISA kit has been designed to allow rapid screening and quantification of mouse NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.

This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, thus allowing easy progression into the larger individual assay sizes when needed. The Multi-Neurotrophin RapidTM Screening ELISA kit therefore presents a cost effective way to quickly screen multiple samples, which can then be published or used prior to a more extensive analysis with the individual kits. The kit comes complete with all detection reagents and is ready to use. Individual coated strips are provided and each set of standards and detection antibodies are color-coded.

NOTE: For research use only. Not for diagnostic and clinical purposes.
Related products BEK-2211 (Rapid BDNF ELISA)BEK-2213 (Rapid Mouse NGF ELISA)BEK-2218 (Rapid NT4/5 ELISA)BEK-2221 (Rapid NT3 ELISA)
Batch No. Refer to the product label.
Antigen The Neurotrophin family of growth factors in all mammals including human has four members including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin 3 (NT3) and Neurotrophin 4/5 (NT4/5). These are all essential to brain development, maturation and adult function, particularly for cell differentiation, survival, death and synaptic regulation.
Specificity This Multi-Neurotrophin Screening kit uses the same antibodies as in the individual Rapid ELISA kits. Each kit has been tested for cross-reactivity with other closely related neurotrophins and no cross-reactivity has been observed at 25 ng/mL of each neurotrophin tested.
Cross-reactivity The antibodies used in this kit detect the human and rat form of each neurotrophin, but with varying efficiency depending on the target protein.
Storage Store at 2-8C
Expiry Date 12 months from purchase.
Specific References Noda T et al. (2019) Effects of Tokishakuyakusan on regeneration of murine olfactory neurons in vivo and in vitro. Chem Senses. [Epub ahead of print]. Application: Mouse olfactory bulb homogenate.
Kit components The ELISA kit box contains 2 x 96-well pre-coated strip plates (6 strips/48 wells per neurotrophin target), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Range NGF (3.9 - 250 pg/mL); BDNF (7.8 - 500 pg/mL); NT3 (15.6 - 1000 pg/mL); NT4/5 (15.6 - 1000 pg/mL)
Sensitivity Typical LODs achieved based on 150% of background OD: NGF: 3 pg/mL BDNF: 1 pg/mL NT3: 4 pg/mL NT4/5: 10 pg/mL
Kit protocol Click to download the kit protocolBEK-2231_as_at_May2018.pdf
MSDS Click to download the Material Safety Data SheetMSDS_Rapid_ELISA_as_at_Aug2017.pdf
Images (click to zoom)
Multi-Neurotrophin Rapid Screening ELISA Kit: Mouse These standard curves generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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