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Neurotrophin 4/5 (NT4/5) Rapid ELISA Kit: Human, Mouse, Rat and Monkey (2 Plates)

$767.00USD


Catalogue No. BEK-2218-2P
  Find a 1 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com
Description The Biosensis Neurotrophin 4/5 RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT4/5 in less than 4 hours in cell culture supernatants, human citrate-plasma and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples. Accurate quantification of NT4/5 in human citrate-plasma requires a heterophilic antibody (HA) blocker which can be purchased separately (BL-003-1000).

This ELISA kit consists of a pre-coated polyclonal anti-NT4/5 capture antibody, a biotinylated anti-NT4/5 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT4/5 present in samples and protein standards.

This NT4/5 ELISA kit employs a recombinant human NT4/5 standard. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey.

The antibodies used in this kit bind to epitopes within the mature domain of NT4/5. Thus, this ELISA kit will quantify the mature form of NT4/5, and may also detect the full-length pro-form of NT4/5.

This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Related products BL-003-1000, Heterophilic antibody blocker: Sample diluent additive for accurate quantification or NT4/5 in human citrate-plasma.
Batch No. Refer to the product label.
Antigen FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Other Names Neurotrophin-5; NT-5; Neutrophic factor 5; Neurotrophin-4; NT-4; Neutrophic factor 4; NTF5; NTF4
Accession NTF4_HUMAN
Specificity Human.
Cross-reactivity The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey.
Storage Store at 2-8C.
Expiry Date 12 months from purchase.
Specific References Allen RS et al. (2018) TrkB signaling pathway mediates the protective effects of exercise in the diabetic rat retina. Eur J Neurosci. doi: 10.1111/ejn.13909. [Epub ahead of print]. Application: Rat retina homogenates.

Maejima H et al. (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates.

Takahashi K et al. (2016) Exercise combined with low-level GABAA receptor inhibition up-regulates the expression of neurotrophins in the motor cortex. Neurosci Lett. doi: 10.1016/j.neulet.2016.10.052. [Epub ahead of print]. Application: Mouse cortex homogenates, in native cell lysis buffer.
General References Lodouichi (2000) J. Neuroscie. 20(6): 2155
Zhang et al. (1999) J Neurosci. Meths. 89 (1): 69
Kit components The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Range 15.6 - 1000 pg/mL
Sensitivity This ELISA kit typically detects down to 25 pg/mL human NT4/5 (defined as 150% of blank value).
Kit protocol Click to download the kit protocolBEK-2218_as_at_March 2017_Final.pdf
MSDS Click to download the Material Safety Data SheetMSDS_Rapid_ELISA_as_at_Aug2017.pdf
Images (click to zoom)
Neurotrophin 4/5 (NT4/5) Rapid ELISA Kit: Human, Mouse, Rat and Monkey (2 Plates) This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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