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Neurotrophin 3 (NT3) Rapid ELISA Kit: Human, Rat and Mouse (2 Plates)

$767.00USD


Catalogue No. BEK-2221-2P
  Find a 1 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com
Description The Biosensis NT3 RapidTM enzyme-linked immune-sorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT3 in less than 4 hours in cell culture supernatants and human plasma (EDTA and citrate) only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human plasma. Accurate quantification of NT3 in human plasma requires a heterophilic antibody (HA) blocker which can be purchased separately (BL-004-500).

This ELISA kit consists of a pre-coated polyclonal anti-NT3 capture antibody, a biotinylated monoclonal anti-NT3 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT3 present in samples and protein standards. A human NT3 positive control (QC sample) is provided to assure consistent assay performance.

This NT3 RapidTM ELISA kit employs a recombinant human NT3 standard. NT3 is highly conserved and nearly identical in many species. The assay's capture and detection antibodies detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 RapidTM ELISA will also react with those species and possibly more because of the conversation of the immunogen used.

The antibodies used in this kit bind to epitopes within the mature domain of NT3. Thus, this ELISA kit may detect the full-length pro-form of NT3.

This kit has not been tested for other applications. Sufficient amount of NT3 standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Related products BL-004-500, Heterophilic antibody blocker: Sample diluent additive for accurate quantification or NT3 in human plasma (EDTA and citrate).
Batch No. Refer to the product label.
Antigen NT3 is understood to be important in promote the survival of visceral and proprioceptive sensory neurons. It is a secreted protein that belongs to the NGF-beta family and is found in brain and peripheral tissues.
Other Names Neurotrophin-3; Neurotrophic factor; HDNF; Nerve growth factor 2; NGF-2; Ntf3; Ntf-3
Accession NTF3_HUMAN
Specificity Human. The capture and detection antibodies used detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 ELISA will also react with those species.
Storage Store at 2-8°C.
Expiry Date 12 months from purchase.
Specific References Shen W, Yau B, Lee SR, Zhu L, Yam M, Gillies MC (2017) Effects of Ranibizumab and Aflibercept on Human Müller Cells and Photoreceptors under Stress Conditions. Int J Mol Sci. 2017 Mar 1;18(3). pii: E533. doi: 10.3390/ijms18030533. Application: Human cell line supernatants.
Kit components The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Range 15.6 - 1000 pg/mL
Sensitivity This NT3 ELISA kit typically detects a minimum of 5 pg/mL NT3 (defined as 150% of blank value).
Kit protocol Click to download the kit protocolBEK-2221_as_at_Apr2018.pdf
MSDS Click to download the Material Safety Data SheetMSDS_Rapid_ELISA_as_at_Aug2017.pdf
Images (click to zoom)
Neurotrophin 3 (NT3) Rapid ELISA Kit: Human, Rat and Mouse (2 Plates) This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.

PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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