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Oligomeric Human beta-Amyloid Abeta1-42 Peptide, Stabilized

$97.00USD


Catalogue No. PE-1750-1000
Description A proprietary preparation of human amyloid beta peptide (amino acids 1-42) that was initially monomerized by HFIP-treatment and then allowed to form oligomers by the procedure described in Youmans KL et al., 2012, followed by lyophilisation using Biosensis’ proprietary stabilization procedures.

The resulting oligomeric mixture has been specially designed to allow the formation of stable, oligomeric Aβ1-42 peptide, multimeric complexes or oligomers. The material is intended to be used as a stable and consistent standard or positive control for oligomeric ELISA assays, as well as other research applications. 
Related products Oligomeric Aβ ELISA Kit (BEK-2215)
ApoE/Aβ Complex ELISA kit (BEK-2224)
Beta-Amyloid Aβ1-42 Peptide, HFIP-treated (PE-1749-50)
MOAB-2 Mouse Monoclonal Antibody to Aβ Peptide (Aβ1-40/42, M-1586-100)
Batch No. See product label. Each lot is tested by ELISA assay and validated for oligomer formation (Oligomeric Aβ ELISA Kit, BEK-2215).
Unit size 1000 ng. Supplied as 2 x 500 ng vials, each containing lyophilized Aβ oligomers. Note that the amount of provided oligomeric protein is based on the amount of monomeric Aβ used to form these oligomers. The precise formation, size and number of oligomers cannot be quantified by any known method.
Sequence DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
Other Names Beta-APP42; Beta-amyloid protein 42; ABPP; APPI; Amyloid beta A4 protein; AB42; abeta
Accession P05067 A4_HUMAN;
Molecular Weight 4.5 kDa (monomeric Aβ1-42).
Applications Use as positive control in Oligomeric Aβ ELISA Kit (BEK-2215): Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 0.5-1 ng/mL. At this concentration, a positive signal will be obtained within the dynamic range of the calibration curve.

Use as oligomeric A-beta peptide standard in Oligomeric Aβ ELISA Kit (BEK-2215): Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 2 ng/mL, which represents the highest concentration of the calibration curve. Perform a 1:2 serial dilution down to 0.031 ng/mL in assay buffer. Click here for detailed instructions on generating a calibration curve with PE-1750-1000.

Use as positive control in other applications: Optimal concentrations need to be determined empirically. It is recommended to reconstitute the vial with 100 – 200 uL buffer first (eg., PBS, pH 7.4), and prepare further working dilutions thereof.
Appearance Lyophilized powder, without preservatives. May appear wet due to the hygroscopic nature of the stabilizing buffer.
Storage Store unopened, lyophilized oligomeric Aβ with desiccant, insulated, at -20°C short term, -80°C long term. Store reconstituted vial at 2- 8°C for up to 2 days. The reconstituted material should not be frozen for best results.
Expiry Date Stability of unopened vials is 6 months if stored appropriately.
General References Youmans KL. et al. (2012) Intraneuronal Abeta detection in 5xFAD mice by a new Abeta-specific antibody. Mol Neurodegener. 2012 Mar 16;7(1):8.
Images (click to zoom)
Oligomeric Human beta-Amyloid Abeta1-42 Peptide, Stabilized Oligomeric A-beta standard curves generated with BEK-2215 (Oligomeric A beta ELISA kit). Pre-formed oligomers (PE-1750-1000) were reconstituted in assay buffer and compared to oligomers freshly prepared from HFIP-treated A-beta peptide (PE-1749-50). This data demonstrates the usefulness of PE-1750-1000 as oligomeric A-beta protein standard.

PDF Data Sheet

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MSDS

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