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Rabbit antibody to VRL-1 (744-761): affinity purified

$297.00USD


Catalogue No. R-078-50
Description TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
Batch No. See product label
Unit size 50 ug
Antigen A synthetic peptide (C-KNSASEEDHLPLQVLQSP) as part of rat VRL-1 protein (aa: 744-761) conjugated to KLH has been used as the immunogen.
Other Names osm-9-like TRP channel 2; OTRPC2
Accession VRL_RAT
Produced in Rabbit
Purity Affinity Purified.
Applications IHC, Immunofluorescence, Western blot and Immunoblot. Recommended to be used at a concentration of 0.5-1 ug for these applications. This antiserum works superbly for immunohistochemistry on free-floating or paraffin embedded sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Immunohistochemical analysis in rat dorsal root ganglia and spinal cord indicates a high level of specificity for this antiserum. Specificity was also shown by Western blot.
Cross-reactivity This antibody is known to react with rat and human VRL-1.
Blast it If you would like to use this product in another species other than those specified here, or to see the shared ID between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Lyophilised from PBS, pH 7.4, containing 0.02% sodium azide as preservative.
Reconstitution Reconstitute in 50 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent.
Expiry Date 12 months after purchase
References 1. Caterina, et al., Nature 398, 436-41 (Apr 1, 1999).
2. Jahnel, et al., Eur J Biochem 270, 4264-71 (Nov, 2003).
3. Montell, et al., Mol Cell 9, 229-31 (Feb, 2002).
MSDS Click to download the Material Safety Data SheetMSDS_R-078-50_as_at_July2016.pdf
Images (click to zoom)
Rabbit antibody to VRL-1 (744-761): affinity purified Immunohistochemical staining of Vanilloid Receptor Like Protein (VRL-1) in rat trigeminal ganglion using rabbit polyclonal antibody to rat VRL-1, catalogue number R-078-50.
Rabbit antibody to VRL-1 (744-761): affinity purified Immunohistochemical staining of Vanilloid Receptor Like Protein (VRL-1) in rat dorsal horn using rabbit polyclonal antibody to rat VRL-1, catalogue number R-078-50.
Rabbit antibody to VRL-1 (744-761): affinity purified Western blot of VRL-1 in PC12 cell lysate (80 μg/lane). R-078-50 detects glycosylated VRL-1 monomer at around 90-95 kDa. VRL-1 dimer is also detected by this antibody in this cell lysate preparation. SDS-PAGE: denatured and non- reduced; Transfer: Tris-Glycine buffer; Membrane: nitrocellulose (0.45 μm); Blocking: 5% skim milk in TBST, 2 hours at RT; Primary antibody: overnight at 4C (2 μg/mL); Secondary antibody: anti-rabbit-HRP (1/6000), 1 hour at RT; Detection: Chemiluminiscence
PDF Data Sheet

Click to download the data sheet - Click to download the Data Sheet

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