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Rabbit antibody to GFP: affinity purified


Catalogue No. R-1838-100
Description The green fluorescent protein (GFP) is a 27 kDa protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395nm and emission maximum at 509 nm, which is similar to that of fluorescein. GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell.
This GFP rabbit antibody was made against a recombinant GFP construct originating from an Aequoria species which was engineered to improve spectral properties and prevent oligomerization (1). This form of GFP, referred to as AcGFP, is 94% identical to the eGFP developed by Tsien and co-workers. The antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.
Batch No. See product label
Unit size 100 ug
Antigen Recombinant AcGFP purified from E. coli
Antibody Type Rabbit polyclonal
Accession Q6YGZ0_9CNID
Produced in Rabbit
Molecular Weight ~ 27 kDa
Purity Affinity purified
Applications Immunocytochemistry (1:2,000 - 1:5,000) and Western Blot (1:1,000 - 1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity GFP
Species Against Species independent
Antibody Against GFP
Form Lyophilized from a solution containing PBS, pH 7.2-7.6, 0.1% trehalose, 5 mM sodium azide as preservative.
Reconstitution Reconstitute with 100 uL sterile-filtered, ultrapure water, to achieve an antibody concentration of 1 mg/mL. Centrifuge briefly to remove any insoluble material.
Storage Store lyophilized antibody at 2-8C. After reconstitution divide into aliquots and store at -20C for long-term storage. Store at 2-8C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase if unopened
General References 1. Gurskaya NG et al. (2003) A colourless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutants. Biochem J. 373(Pt 2):403-8.
Images (click to zoom)
Rabbit antibody to GFP: affinity purified Left: Analysis of GFP expression (green) in transfected HEK293 cells by Immunocytochemistry. Cells were stained with rabbit anti-GFP antibody (red, 1:2,000). Blue: Hoechst staining for nuclei. Transfected cells appear orange-yellow due to the green (GFP) and red (anti-GFP antibody) color overlay. Right: Western blot analysis of GFP protein in transfected HEK293 lysate using rabbit antibody to GFP (green, 1:2,000). [1] protein standard, [2] non-transfected control cells, [3] transfected cells with GFP construct, [4] transfected cells with mCherry construct. A strong band at ~27 kDa corresponds to the expected molecular weight of GFP protein detected only in cells transfected with GFP construct. The antibody does not cross-react with mCherry protein. The same blot was simultaneously probed with a mouse antibody to beta-tubulin (red, ~ 50 kDa, lanes 2-4).

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