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Rabbit antibody to ATG12: IgG

$297.00USD


Catalogue No. R-137-500
Description FUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the ATG12 family.
Batch No. See product label
Unit size 500 ug
Antigen A synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Other Names Autophagy-related protein 12; APG12-like; ATG12; APG12; APG12L
Accession ATG12_HUMAN
Produced in Rabbit
Purity Protein G purified IgG
Applications IHC, immunofluorescence, WB (1-5 ug/mL working concentration), Flow Cytometry (2ug/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity IHC and WB confirmed the specificity for ATG12.
Cross-reactivity Human, rat
Form Lyophilised
Reconstitution Reconstitute in 500 uL of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
References 1. Mizushima, N. et al. J. Biol. Chem. 273(51):33889-33892 (1998).
2. Tanida, I. et al. Biochem. Biophys. Res. Commun. 296(5):1164-1170 (2002).
3. Tanida, I. et al. J. Biol. Chem. 277(16):13739-13744 (2002).
Images (click to zoom)
Rabbit antibody to ATG12: IgG Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 15% Horse serum, Primary antibody: Rabbit Polyclonal antibody to ATG12 (cat # R-137-500, 2μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Donkey anti-rabbit PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Normal Rabbit Polyclonal IgG was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols. The data demonstrates specific staining of ATG12 expressed in human neuroblastoma SH-SY5Y cell line using cat # R-137-500.

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