||ATG16 L1 plays an essential role in autophagy. It is a homooligomer ant interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). APG16 L1 localises to the cytoplasm, in particularly to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. There are 5 named isoforms produced by alternative splicing. Genetic variation within ATG16 L1 are associated with susceptibility to inflammatory bowel disease type 1.
||See product label
||N-terminal of human ATG16-L1 protein conjugated to Blue Carrier Protein has been used as the immunogen.
||Autophagy-related protein 16-1; APG16-like 1; ATG16L1
||Non-reduced western blot: 1:100; IP: 1-50-1:100 (5-10uL/500uL lysate); ELISA: 1:2000-1:3000 direct detection only, recombinant immunogen.
||Detects human protein via non-reduced WB, and ELISA
||Human, rat. Other species not yet tested.
||Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
||After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
||Six months after purchase
||1. Cooney R et al (2009). NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation. Nat. Med. Dec 6 2009
2. Scharl M. et al (2011). Protein tyrosine phosphatase nonreceptor type 2 regulates autophagosome formation in human intestinal cells. Inflamm Bowel Dis. 2011 Oct 10.
||1. Zheng H, et al. DNA Seq. 15:303-305 (2004).
2. Clark H.F, et al. Genome Res. 13:2265-2270 (2003).