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Rabbit antibody to ATG16 L1: whole serum

$297.00USD


Catalogue No. R-158-100
Description ATG16 L1 plays an essential role in autophagy. It is a homooligomer ant interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). APG16 L1 localises to the cytoplasm, in particularly to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. There are 5 named isoforms produced by alternative splicing. Genetic variation within ATG16 L1 are associated with susceptibility to inflammatory bowel disease type 1.
Batch No. See product label
Unit size 100 l
Antigen N-terminal of human ATG16-L1 protein conjugated to Blue Carrier Protein has been used as the immunogen.
Other Names Autophagy-related protein 16-1; APG16-like 1; ATG16L1
Accession A16L1_HUMAN
Produced in Rabbit
Purity Whole serum
Applications Non-reduced western blot: 1:100; IP: 1-50-1:100 (5-10uL/500uL lysate); ELISA: 1:2000-1:3000 direct detection only, recombinant immunogen.
Specificity Detects human protein via non-reduced WB, and ELISA
Cross-reactivity Human, rat. Other species not yet tested.
Form Lyophilised
Reconstitution Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 4C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Expiry Date Six months after purchase
Specific References 1. Cooney R et al (2009). NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation. Nat. Med. Dec 6 2009
2. Scharl M. et al (2011). Protein tyrosine phosphatase nonreceptor type 2 regulates autophagosome formation in human intestinal cells. Inflamm Bowel Dis. 2011 Oct 10.
References 1. Zheng H, et al. DNA Seq. 15:303-305 (2004).
2. Clark H.F, et al. Genome Res. 13:2265-2270 (2003).
Images (click to zoom)
Rabbit antibody to ATG16 L1: whole serum

Atg16 L1 detected in cytospun HL60 cells by fluorescence confocal microscopy using antibody R-158-100. HL60 cells were fixed with acetone 90 seconds; primary antibody was diluted 1:100 and incubated for 1 hour, 3 x washes and secondary (Goat anti Rb 1:100 alexa 546 conjugate) for 30 minutes. Cells stained for Atg16 L1 appear in red. The cells were then counter-stained with Hoechst Dyeblue colour.

Rabbit antibody to ATG16 L1: whole serum Western blot (non-reducing) on Jurkat cell lysate using Rabbit antibody to ATG16 L1: whole serum (R-158-100).
Rabbit antibody to ATG16 L1: whole serum Western blot (non-reduced) on Peripheral Blood Mononuclear Cells (PMBC) lysate using Rabbit antibody to ATG16 L1: whole serum (R-158-100) at a dilution of 1: 100 (ECL).
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MSDS

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