Rabbit antibody to ATG4C (APG4C): whole serum

$297.00USD

Catalogue No.	R-156-100
Description	FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable).? SIMILARITY: Belongs to the peptidase C54 family.
Batch No.	See product label
Unit size	100 uL
Antigen	A synthetic peptide (CTVYNSDVIDKQ) corresponding to the C-terminal of human ATG4C protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from ATG4C protein in mouse and rat.
Other Names	Cysteine protease ATG4C; Autophagy-related protein 4 homolog C; Autophagin-3; Autophagy-related cysteine endopeptidase 3; AUT-like 3 cysteine endopeptidase; ATG4C; APG4C; AUTL3;
Accession	ATG4 C_HUMAN ATG4 C_MOUSE ATG4 C_RAT
Produced in	Rabbit
Purity	Whole serum
Applications	IHC, immunofluorescence and WB. A dilution of 1:100 to 1:2000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity	IHC, ELISA and WB confirmed the specificity for ATG4C.
Cross-reactivity	Human, rat. Other species not yet tested.
Form	Lyophilised
Reconstitution	Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material.
Storage	After reconstitution keep aliquots at -20C for a higher stability, and at 2-8C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date	Six months after purchase
Specific References	Smuder AJ et al. (2011) Exercise protects against doxorubicin-induced markers of autophagy signaling in skeletal muscle. J Appl Physiol (1985). 2011 Oct;111(4):1190-8.
General References	1. Marino G, et al. J. Biol. Chem. 278:3671-3678(2003). 2. Ota T, et al. Nat. Genet. 36:40-45(2004).

Images (click to zoom)

Confocal microscopy on immunofluorescently detected APG4C in HL60 cell line using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for APG4C appear in red. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Note: More IHC analysis is underway. Please enquire.

	Western blot (non-reducing) on Jurkat cell lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100).
	Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1: 100 (ECL).
	Western blot under reducing conditions on Olfactory cell line (Odora) lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1:100.
	Western blot under reducing conditions on rat liver lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1:100.
	Western blot under reducing conditions on 293 cell lysate using Rabbit antibody to ATG4C (APG4C): whole serum (R-156-100) at a dilution of 1:100.

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