Catalogue No. |
R-156-100 |
Description |
FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). SIMILARITY: Belongs to the peptidase C54 family. |
Batch No. |
See product label |
Unit size |
100 µl |
Antigen |
A synthetic peptide (CTVYNSDVIDKQ) corresponding to the C-terminal of human ATG4C protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from ATG4C protein in mouse and rat. |
Other Names |
Cysteine protease ATG4C; Autophagy-related protein 4 homolog C; Autophagin-3; Autophagy-related cysteine endopeptidase 3; AUT-like 3 cysteine endopeptidase; ATG4C; APG4C; AUTL3; |
Accession |
ATG4 C_HUMAN
ATG4 C_MOUSE
ATG4 C_RAT |
Produced in |
Rabbit |
Purity |
Whole serum |
Applications |
IHC, immunofluorescence and WB. A dilution of 1:100 to 1:2000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
Specificity |
IHC, ELISA and WB confirmed the specificity for ATG4C. |
Cross-reactivity |
Human, rat. Other species not yet tested. |
Form |
Lyophilised |
Reconstitution |
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
Storage |
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. |
Expiry Date |
Six months after purchase |
Specific References |
Smuder AJ et al. (2011) Exercise protects against doxorubicin-induced markers of autophagy signaling in skeletal muscle.
J Appl Physiol (1985). 2011 Oct;111(4):1190-8.
|
General References |
1. Marino G, et al. J. Biol. Chem. 278:3671-3678(2003).
2. Ota T, et al. Nat. Genet. 36:40-45(2004). |