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Rabbit antibody to ATG7: whole serum

$297.00USD


Catalogue No. R-161-100
Description FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. SIMILARITY: Belongs to the ATG7 family. In yeast, ATG7 appears to be required for fusion of peroxisomal and vaculuolar membranes.
Batch No. See product label
Unit size 100 µl
Antigen A synthetic peptide (DSTRDRTLDQQC) corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG7 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Other Names Autophagy-related protein 7; Ubiquitin-activating enzyme E1-like protein; hAGP7; APG7-like; ATG7; APG7L
Accession ATG7_HUMAN
ATG7_MOUSE
ATG7_RAT
ATG7_Saccharomyces cerevisiae
ATG7_Macaca mulatta
ATG7_Canis familiaris
Produced in Rabbit
Purity Whole serum
Applications Non-reduced western blot : 1:100; IF: acetone or 3.7% PFA fixed cells (HJ Lim et al 2013); 1:100; Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity IF, WB and ELISA confirmed the specificity for ATG7.
Cross-reactivity Human, rat. Other species not yet tested.
Form Lyophilised
Reconstitution Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
Storage Store lyophilized antibody at 2-8ºC. After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
Specific References Chiu-Wei Chen, et al (2012) Inhibition of autophagy as a therapeutic strategy of iron-induced brain injury after hemorrhage Autophagy, 8(10):1510.
Chien W.S. et al (2011) Suppression of autophagy in rat liver at late stage of polymicrobial sepsis Shock. 2011 Jan 14
Lee J.E. et al (2011) Autophagy Regulates Embryonic Survival During Delayed Implantation Endocrinology. 2011 Mar 1.
Ryningen A et al (2012) Inhibition of Mammalian target of rapamycin in human acute myeloid leukemia cells has diverse effects that depend on the environmental in vitro stress. Bone Marrow Res. 2012;2012:329061.
Oh HA et al (2013) Uncovering a role for endocannabinoid signaling in autophagy in preimplantation mouse embryos. Mol Hum Reprod. 2013 Feb;19(2):93-101.
References 1. Yuan W, et al. Mol. Biol. Cell 10:1353-1366(1999).
2. Tanida I, et al. Biochem. Biophys. Res. Commun. 292:256-262(2002).
3. Tanida I, et al. J. Biol. Chem. 277:13739-13744(2002).
4. Mizushima N, et al. Nature 395:395-398(1998).
5. Johnston M, et al. Science 265:2077-2082(1994).
6. Harding T.M, et al. J. Cell Biol. 131:591-602(1995).
7. Kim J, et al. Mol. Biol. Cell 10:1337-1351(1999).
8. Komatsu M, et al. J. Biol. Chem. 276:9846-9854(2001).
9. Klionsky D.J, et al. Dev. Cell 5:539-545(2003).
10. chimura Y, et al. J. Biol. Chem. 279:40584-40592(2004).
Images (click to zoom)
Rabbit antibody to ATG7: whole serum Confocal microscopy on cytospin-isolated human blood cells. Neutrophils seem to be stained using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG7 appear in red. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
Observations:
No staining was observed using the Pre-immunisation serum. No staining was observed in HL60 cell line.

Rabbit antibody to ATG7: whole serum Western blot on Peripheral Blood Mononuclear Cells, non-reduced, (PMBC) lysate using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1:100 (ECL).
Rabbit antibody to ATG7: whole serum Confocal microscopy on immunofluorescently detected APG7 in cytospin-isolated human white blood cells using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells were double-stained for APG7 appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented.
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MSDS

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