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Rabbit antibody to ATG9A (APG9L1): whole serum

$297.00USD


Catalogue No. R-160-100
Description FUNCTION: Plays a role in autophagy. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (Potential). ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the ATG9 family. IN YEAST: FUNCTION: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Recruites ATG23 and ATG8 to the pre-autophagosomal structure. SUBUNIT: Interacts with ATG18, ATG2 and ATG23. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. Preautophagosomal structure; preautophagosomal structure membrane; multi-pass membrane protein. Note=Pre-autophagosomal and other perivacuolar punctate structures. The proper trafficking of ATG9 between the pre-autophagosomal structure and the other punctate structures requires ATG2, ATG18, ATG23, the ATG1-ATG13 complex and the phosphatidylinositol 3-kinase complex I. SIMILARITY: Belongs to the ATG9 family.
Batch No. See product label
Unit size 100 l
Antigen A synthetic peptide (CWHRRESDESGE) corresponding to the C-terminal of human APG9A (APG9L1) protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG9A (APG9L1) protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Other Names Autophagy-related protein 9A; APG9-like 1; Autophagy-related protein 9; APG9A; ATG9A; APG9L1
Accession ATG9 L1_HUMAN
ATG9 L1_MOUSE
ATG9 L1_RAT
ATG9 L1_Saccharomyces cerevisiae
ATG9 L1_Macaca mulatta
ATG9 L1_Canis familiaris
Produced in Rabbit
Purity Whole serum
Applications IHC, immunofluorescence, WB. A dilution of 1:100 to 1:3000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity IHC, WB and ELISA confirmed the specificity for ATG9A (APG9L1) .
Cross-reactivity Human, rat. Other species not yet tested.
Form Lyophilised
Reconstitution Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 4C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date Six months after purchase
Specific References 1. Park YE et al (2009) Autophagic degradation of nuclear components in mammalian cells. Autophagy. 2009 Aug;5(6):795-804.
References 1. Ota T, e al. Nat. Genet. 36:40-45(2004).
2. Delaveau T.T.D, et al. Yeast 12:1587-1592(1996).
3. Jacq C, et al. Nature 387:75-78(1997).
4. Tsukada M., Ohsumi Y. FEBS Lett. 333:169-174(1993).
5. Harding T.M, et al. J. Cell Biol. 131:591-602(1995).
6. Harding T.M, et al. J. Biol. Chem. 271:17621-17624(1996).
7. Lang T, et al. J. Bacteriol. 182:2125-2133(2000).
8. Noda T, et al. J. Cell Biol. 148:465-480(2000).
9. Kim H, et al. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
10. Tucker K.A, et al. J. Biol. Chem. 278:48445-48452(2003).
Images (click to zoom)
Rabbit antibody to ATG9A (APG9L1): whole serum Confocal microscopy on mouse lymph node using Rabbit antibody to ATG9A (APG9L1): whole serum (R-160-100).
Rabbit antibody to ATG9A (APG9L1): whole serum Western blot (non-reducing) on Jurkat cell lysate using Rabbit antibody to ATG9A (APG9L1): whole serum (R-160-100).
Rabbit antibody to ATG9A (APG9L1): whole serum Western blot on Peripheral Blood Mononuclear Cells (PBMC) lysate using Rabbit antibody to ATG9A (APG9L1): whole serum (R-160-100) at a dilution of 1: 100 (ECL).
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