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Rabbit antibody to BDNF (129-138): affinity purified

$227.00USD


Catalogue No. R-172-20
Description
This product is no longer available. Please see below for our recommended alternative products.
Replaced by Mouse monoclonal antibody to rhBDNF, Clone 4C8, IgG, cat# M-1744-50/100
Rabbit polyclonal antibody to rhBDNF, affinity-purified, cat# R-1707-100
Rabbit polyclonal antibody to rhBDNF, whole serum, cat# R-088-100
Rabbit polyclonal antibody to rhBDNF, IgG, cat# R-017-500
Rabbit polyclonal antibody to BDNF (129-138), whole serum, cat# R-083-100
Rabbit polyclonal antibody to BDNF (129-138), IgG, cat# R-066-100
Batch No. See product label
Unit size 20 g
Antigen A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst many mammalian species.
Other Names Brain-derived neurotrophic factor; Abrineurin; proBDNF;
Accession BDNF_HUMAN
Produced in Rabbit
Purity Affinity purified
Applications IHC, ELISA. A working concentration of 1-10 g/ml is recommended for IHC and ELISA. We suggest R-083-100 or R-084-100 for western blotting applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum also easily recognises recombinant human BDNF in western blot.
Cross-reactivity This antiserum is known to recognise rat, mouse and human BDNF.
Blast it If you would like to use this product in another species other than those specified here, or to see the shared ID between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Lyophilised
Reconstitution Reconstitute in 20 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 4C with an appropriate antibacterial agent. Glycerol (1:) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
Specific References
Moses, C et al. (2015) The Acquisition of Target Dependence by Developing Rat Retinal Ganglion Cells. eNeuro:2 (3) Species: Rat. RDG cell cultures; Application: ICC/IF; 4% PFA fixed, 0.2% triton treated.
References 1. A Acheson et al (1995) Nat. 74: 450-3
2. Q Yan et al (1994) J. Neurosci. 14(9): 5281-91
3. XF Zhou et al (1996) Neurosci. 74: 945-53
4. XF Zhou, et al (1998) Exp. Neurol. 149: 237-42
5. B Mellstrom et al (2004) Crit Rev Neurobiol 16, 43-9
6. I Tapia-Arancibia et al (2004) Front Neuroendocrinol 25, 77-107
7. S Pezet, et al (2002) Brain Res Brain Res Rev 40, 240-9
8. Barde Y. A. et al (1989) EMBO J. 1: 549
9. Conner J et al. (1997) J. Neurosci. 17: 2295
10. JA Coull et al (2005) Nature. Dec 15;438(7070):1017-21.
11. C Gomes et al (2013) J Neuroinflammation. Jan 30;10:16.
Images (click to zoom)
Rabbit antibody to BDNF (129-138): affinity purified The rat spinal cord section was stained with Rabbit antibody to BDNF (129-138): affinity purified (R-172-20), at a concentration of 2 µg/ml. Nerve terminals in the dorsal horn are labelled. Secondary detection with CY3 labelled antibodies. (Image courtesy of KJ Wong).
Rabbit antibody to BDNF (129-138): affinity purified Section dorsal root ganglion from adult rat showing the presence of BDNF immunoreactivity in small diametre neurons and associated nerve fibres. Rabbit atnibody to BDNF (129-138): affinity purified (R-172-20), was used at 2 g/ml. Secondary detection with CY3 labelled antibodies. (Image courtesy of KJ Wong).
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MSDS

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