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Rabbit antibody to BDNF (129-148): whole serum

$297.00USD


Catalogue No. R-084-100
Description
This product is no longer available. Please see below for our recommended alternative products.
Replaced by Mouse monoclonal antibody to rhBDNF, Clone 4C8, IgG, cat# M-1744-50/100
Rabbit polyclonal antibody to rhBDNF, affinity-purified, cat# R-1707-100
Rabbit polyclonal antibody to rhBDNF, whole serum, cat# R-088-100
Rabbit polyclonal antibody to rhBDNF, IgG, cat# R-017-500
Rabbit polyclonal antibody to BDNF (129-138), whole serum, cat# R-083-100
Rabbit polyclonal antibody to BDNF (129-138), IgG, cat# R-066-100
Batch No. See product label
Unit size 100 l
Antigen A synthetic peptide (HSDPARRGELSVCDSISEWV) as a part of human BDNF protein (aa: 129-148) conjugated to KLH has been used as the immunogen. This sequence is highly conserved amongst mammals.
Other Names Brain-derived neurotrophic factor; Abrineurin; proBDNF;
Accession P23560 BDNF_HUMAN;
Produced in Rabbit
Purity Whole serum
Applications WB. A dilution of 1:500 to 1:2000 is recommended. For IHC applications, we recommend R-172-20. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity This antiserum specifically recognises recombinant BDNF and also detects a ~35 kDa band corresponding to the molecular weight of proBDNF in rat brain extract. No cross reactivity with NGF, NT3 or NT4/5 was detected by western blot.
Cross-reactivity This antiserum is known to react rat, human and mouse BDNF.
Blast it If you would like to use this product in another species other than those specified here, or to see the shared ID between the immunogen used here in different species and/or other molecules, simply copy the immunogen (from the Immunogen field) and paste it HERE and blast/format it. Note that, antisera raised against synthetic peptides are quite often very specific for that peptide ie, only one single amino acid difference may be enough to restrict the specificity to a particular molecule. Regardless, you can always contact us if you need assistance with this.
Form Lyophilised
Reconstitution Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.
Storage After reconstitution keep aliquots at -20C for a higher stability, and at 4C with an appropriate antibacterial agent. Glycerol (1:) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Expiry Date 12 months after purchase
Specific References 1. Puerta E. et al (2010) Sildenafil protects against 3-nitropropionic acid neurotoxicity through the modulation of calpain, CREB, and BDNF Neurobiol Dis. 2010 May;38(2):237-45.
References BDNF Antibodies for Western Blotting
Images (click to zoom)
Rabbit antibody to BDNF (129-148): whole serum Denatured and reduced mature BDNF was electrophoresed on 12% Bis-Tris gel and transferred onto PVDF membrane (0.22 um) using Dunns carbonate transfer buffer. Membrane was blocked with 5% skim milk in TBST and BDNF detected with R-084-100 (1:1000, overnight incubation) and a secondary anti-rabbit-HRP conjugate (1:6000, 1 hour at room temperature).

PDF Data Sheet

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MSDS

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